مطالعه انگشت‌نگاری ژنومی درون‌رایانه‌ای سویه‌های سیانوباکتریایی بیماری‌زا جداشده از تالاب آلماگل (استان گلستان) با استفاده از توالی‌های به شدت تکراری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد بخش زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 دانشجوی کارشناسی ارشد بخش زیست‌‌‌‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

3 استادیار بخش زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

4 دانشیار بخش زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

10.22092/bot.j.iran.2025.369416.1420

چکیده

تالاب آلماگل (استان گلستان)، محل دایمی پرورش بسیاری از ماهی‌ها و موجودات آبزی، محل بازدید عمومی بسیاری از گردشگران در فصول گرم سال است. شناسایی اجساد مرده چندین ماهی روی سطح آب نشان از حضور سیانوباکتری‌های بیماری‌زا (سیانوتوکسین‌ها) در این منطقه بود. به همین دلیل در تحقیق حاضر، با تکثیر ژن‌های بیماری‌زای mcyG، mcyD و mcyE و همچنین ژن‌های ساختاری از جمله 16S rRNA و ITS به همراه استفاده از روش rep-PCR برای تکثیر ژن‌های به شدت تکراری HIP، STRR و ERIC، به غربالگری سویه بالقوه سیانوباکتری‌های بیماری‌زای این تالاب پرداخته شد. نتایج به دست آمده از دندروگرام حاصل از تکثیر پرایمرهای پالیندرومیک نشان داد سویه‌های Nostoc sp. 9، Aliinostoc sp. 1 و Desmonostoc sp. 10 در کلاد مشترک و سویه‌های Calothrix sp. 7 و Neowestiellopsis sp. 2 در بیشتر موارد، انشعابات جداگانه‌ای را تشکیل دادند. با این حال، تنها سویه Neowestiellopsis sp. 2 حاوی ژن بیماری‌زای mcyD بود. همچنین، نتایج حاصل از آنالیز مقایسه طول مناطق ITS نشان داد که طول منطقه و همچنین ساختار مارپیچ‌های D1-D1 و BOX B با سایر سویه‌ها متفاوت بود. مطالعه حاضر جزو نخستین تحقیقات انجام شده است که نشان داد می‌توان با استفاده از روش انگشت‌نگاری، اطلاعات جدیدی را در زمینه سویه‌های مختلف سیانوباکتری ارایه نمود.

کلیدواژه‌ها

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