تنوع ژنتیکی و ریخت‌شناختی در جمعیت‌های ایرانی Medicago polymorpha با استفاده از آنالیز ریخت‌شناسی و نواحی ITS

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری گروه زیستشناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

2 استاد پژوهش مؤسسه تحقیقات جنگل‌ها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، صندوق پستی 116-13185، تهران، ایران

3 استاد پژوهش گروه علوم و تکنولوژی، مؤسسه کشاورزی و کشاورزی-غذایی کانادا، اتاوا، کانادا

4 دانشیار گروه زیستشناسی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران

5 دانشیار گروه زیستشناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران

چکیده

با توجه به اهمیت روزافزون تغذیه دام با علوفه با ارزش، مطالعه اخیر در مناطق مختلف ایران، با هدف توصیف تنوع ژنتیکی و ریخت‌شناختی جمعیت‌های یونجه (Medicago polymorpha L.) با استفاده از صفات ریخت‌شناسی و تجزیه و تحلیل نواحی فاصله‌انداز داخلیITS) ) انجام گردید. بررسی ریخت‌شناسی 14 جمعیت جمع‌آوری شده از سه منطقه کشور در خرداد 1396 و تجزیه و تحلیل فیلوژنتیک ماکسیمم پارسیمونی و بایسین ناحیه ITS آن‌ها انجام شد. نمونه‌های این گونه از جنبه ریخت‌شناسی به گروه‌های مختلفی دسته‌بندی شدند. طبق نتایج به دست آمده در مطالعه حاضر، میزان بالایی از تنوع در بسیاری از ویژگی‌های ریخت‌شناسی بین جمعیت‌ها مشاهده شد. تجزیه و تحلیل خوشه‌بندی براساس اطلاعات ریخت‌شناسی ارتباط نسبی بین توزیع جغرافیایی را نشان داد. تجزیه و تحلیل فیلوژنتیک براساس داده‌های آی.تی.اس. نشان داد که تمام نمونه‌های M. polymorpha در یک شاخه تک‌نیا تجمیع شدند. مجموع نتایج یافته‌های این مطالعه، حاکی از وجود تنوع زیاد در نمونه‌های M. polymorpha در مناطق مختلف ایران است. به  علاوه، استفاده از صفات ریخت‌شناختی همراه با تجزیه و تحلیل آی.تی.اس. برای ردیابی منشا و تکامل گونه مذکور در ایران مفید است.
 
 

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