شناسایی مولکولی بیماری حباب خشک قارچ دکمه‌ای با استفاده از آغازگرهای اختصاصی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار دانشکده علوم، دانشگاه صنعتی جندی شاپور، دزفول، ایران

2 استاد پژوهش، بخش تحقیقات رستنی‌ها، مؤسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران

3 استادیار گروه بیوتکنولوژی، دانشکده کشاورزی، دانشگاه کشاورزی و منابع طبیعی رامین، خوزستان، ایران

چکیده

بیماری حباب خشک (ناشی از قارچ Lecanicilliumfungicola) یکی از مهلک‌ترین بیماری قارچ دکمه‌ای (Agaricus bisporus) در سراسر جهان است.  بسیاری از علایم این بیماری با یکی دیگر از جدی‌ترین بیماری‌های قارچ دکمه‌ای (بیماری حباب تر ناشی از Mycogone perniciosa) مشابه است. تشخیص سریع و به موقع  می‌تواند نقش تعیین‌کننده‌ای در مدیریت این بیماری ایفا نماید. برای تشخیص اختصاصی L. fingicola یک جفت آغازگر با توانایی تکثیر دو باند 650 و 800 جفت بازی از ناحیه  rDNA طراحی شد که در هر مرحله‌ای از توسعه بیماری قادر به تشخیص آن می‌باشد. در این تحقیق همچنین، روش ساده‌ای برای استخراج مستقیم دی.ان.آ. از کلاهک‌های قارچ دکمه‌ای ابداع شد و تاثیر پیش تیمار بافت کلاهک بر کارایی این روش مورد بررسی قرار گرفت. به علاوه، گونه Simplicillium lamellicola  با استفاده از آغازگرهای عمومی ناحیه آی.تی.اس. برای قارچ‌ها از ایران گزارش شده است.

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