جداسازی و شناسایی قارچ‌های بیمارگر حشرات از خاک‌های زراعی و غیرزراعی در استان کرمانشاه

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران

2 استادیار بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران

3 استادیار حشره‌شناسی کشاورزی، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران

چکیده

در راستای شناسایی قارچ‌های بیمارگر حشرات (انتوموپاتوژن‌ها)، از خاک‌های زراعی و غیرزراعی استان کرمانشاه نمونه‌‌برداری صورت گرفت. در مجموع، 41 نمونه خاک از عمق ۱۵-5 سانتی‌متری خاک از 14 شهرستان‌ استان کرمانشاه جمع‌آوری گردید. در نهایت، 114 جدایه قارچی از خاک‌های مختلف جداسازی شد که بیش‌ترین جدایه‌ها به ترتیب از خاک‌های جنگل، مزرعه، مرتع و باغ به دست آمد. با استفاده از ابزار جستجوی BLAST، توالی‌های آی.تی.اس. دی.ان.ای. ریبوزومی به دست آمده با سایر آرایه‌های معتبر مستخرج از بانک ژن مقایسه گردید.
در نهایت، گونه‌های Aspergillus nomius*، Fusarium oxysporum، F. equiseti،Fusarium sp.  ، Penicillium solitum،P. sizovae*
Penicillium sp.، Alternaria chlamydosporigena،Meyerozyma guilliermondii ،Paramyrothecium roridum ،
Chaetomium elatum،Beauveria bassiana ، B. pseudobassiana* وB. tenella*  شناسایی شدند. گونه‌های ستاره‌دار نخستین بار برای فلور قارچی ایران گزارش می‌شوند.
 
 

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