شواهد مولکولی و ریزریخت‌شناختی جنس سس در ایران

نوع مقاله : سیستماتیک و تنوع زیستی گیاهان

نویسندگان

1 دانش آموخته دکتری، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گنبد کاووس، گنبد کاووس، ایران

2 دانش آموخته دکتری، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه قم، قم، ایران

3 دانشیار گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گنبد کاووس، گنبد کاووس، ایران

4 دانش موخته کارشناسی ارشد، گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گنبد کاووس، گنبد کاووس، ایران

5 استادیار گروه زیست شناسی، دانشکده علوم پایه، دانشگاه گنبد کاووس، گنبد کاووس، ایران

چکیده

سس (Cuscuta) تنها جنس انگلی در تیره پیچکیان است. این جنس پراکنش جهانی داشته و اکثر گونه‌های آن در مناطق گرمسیری، نیمه‌گرمسیری و بعضی در مناطق معتدل پراکندگی دارند. در این مطالعه، ویژگی‌های ریزریخت‌شناختی و مولکولی 12 جمعیت از سه گونه سس (C. australis، C. campestris و C. chinensis) مورد بررسی قرار گرفت تا ارزش تشخیصی آن‌ها ارزیابی شود. در کل، هفت ویژگی کمی و دو ویژگی کیفی در گرده انتخاب شده و مورد اندازه‌گیری قرار گرفتند. مهمترین ویژگی‌ها شامل شکل، تزیینات تکتوم، ضخامت اگزین و طول شیار گرده هستند. براساس این تحقیق، شکل و سطح دانه، جدایی C. australis را از دو گونه دیگر مورد تایید قرار داد. همچنین در این تحقیق، با استفاده از نشانگر هسته‌ای (nrDNA ITS)، روابط فیلوژنتیکی در سه گونه از این آرایه بازسازی گردید. داده‌ها توسط آنالیزهای فیلوژنتیکی شامل بیزین، بیشینه درست‌‌نمایی و بیشینه صرفه‌جویی مورد بررسی قرار گرفتند. در آنالیزهای فیلوژنتیکی همه اعضای این سه گونه یک شاخه با حمایت بسیار بالا (PP=1, ML/BS=100/100) تشکیل دادند و به دو گروه اصلی تفکیک شدند (A و B). شاخه A از نمونه‌های گونه C. australis تشکیل شده و دو گونه  C. campestrisو C. chinensis در شاخه B قرار گرفتند. روش شبکه-همسایه، جدایی جمعیت‌های مورد مطالعه را ثابت کرد و نتایج نشان داد که داده‌های ریزریخت‌شناختی و مولکولی، جدایی این گونه‌ها را از هم تایید می‌کنند.

کلیدواژه‌ها

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