Study of genetic variation among some Gossypium spp. genotypes available in Iran using ISSR molecular marker

Document Type : Research Paper

Authors

1 PhD Student, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Prof., Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Research Associate Prof., Cotton Research Department, Tehran Agricultural and Natural Resources Research Center, Agricultural Research, Education and Extension Organization (AREEO), Varamin, Iran

4 Assistant Prof., Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran

Abstract

The current study was conducted to explore genetic variations among several cotton species, including Gossypium hirsutum cultivar Varamin, G. barbadense cultivar T14, G. herbaceum cultivar Arya, hybrid of G. hirsutum cultivar Varamin × G. barbadense cultivar T14 (V×T14), and a hybrid of G. hirsutum cultivar Varamin × G. herbaceum cultivar Arya (V×A) using inter-simple sequence repeats (ISSR). In this study, a total of 60 bands were amplified using four ISSR marker primers. As a result, 57 bands showed polymorphism. The results showed a high level of diversity in the studied varieties. The highest percentage of polymorphism was related to ISSR4 primer with ACACACACACACACACT sequence (100%) and the lowest percentage was related to ISSR1 primer with AGAGAGAGAGAGAGAGC sequence (92.8%). Cluster analysis showed three major groups. The first group consisted of Arya and Varamin, the second group was comprised of V×A, T14 which showed a high genetic similarity indicating T14 originates from Varamin cultivar, and the third group contained V×T14. Overall, the results showed that, ISSR markers can be effectively used to study the genetic diversity of cotton.
 

Keywords

Article Title [Persian]

بررسی تنوع ژنتیکی برخی از ژنوتیپ‌های موجود گونه‌های پنبه در ایران با استفاده از نشانگر مولکولی ISSR

Authors [Persian]

  • زهرا تاجیک خاوه 1
  • علیرضا ایرانبخش 2
  • موسی‌الرضا وفایی تبار 3
  • مصطفی عبادی 4
1 دانشجوی دکتری، گروه زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 استاد پژوهش، گروه زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
3 دانشیار پژوهش، بخش تحقیقات پنبه و گیاهان لیفی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان تهران، سازمان تحقیقات، آموزش و
4 استادیار، گروه زیست‌شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
Abstract [Persian]

پژوهش حاضر، به منظور بررسی تنوع ژنتیکی چند رقم و هیبرید مختلف پنبه شامل Gossypium hirsutum cultivar Varamin،
G. barbadense cultivar T14، G. herbaceum cultivar Arya، هیبریدG. hirsutum cultivar Varamin × G. barbadense cultivar T14 (V×T14) و هیبرید G. hirsutum cultivar Varamin × G. herbaceum cultivar Arya (V×A) با استفاده از نشانگر مولکولی ISSR انجام شد.
در این پژوهش، از چهار آغازگر استفاده شده، تعداد 60 باند ایجاد گردید که از این تعداد، 57 باند، پلی‌مورفیسمی را نشان دادند. نتایج حاصله همچنین درصد بالایی از تنوع را در ارقام مورد مطالعه نشان داد. بالاترین درصد پلی‌مورفیسمی در آغازگرISSR4  با توالی ACACACACACACACACT (100%) و کمترین درصد پلی‌مورفیسمی در آغازگر ISSR1 با توالی AGAGAGAGAGAGAGAGC دیده شد (92%). آنالیز خوشه‌ای، سه گروه بزرگ را نشان داد. گروه اول شامل ارقام آریا و ورامین، گروه دوم شامل ارقام هیبریدی ورامین در آریا وT14  بود که این تشابه ژنتیکی بیانگر این بود که رقم T14 از رقم ورامین اشتقاق یافته و گروه سوم شامل ارقام هیبریدی ورامین در T14 بود. نتایج حاصل از این پژوهش نشان داد که نشانگر مولکولی ISSR می‌تواند به طور مؤثری در مطالعه تنوع ژنتیکی در گیاه پنبه مورد استفاده قرار گیرد.
 
 

Keywords [Persian]

  • پنیرکیان
  • تتراپلویید
  • تنوع ژنتیکی
  • دیپلویید
  • رقم
  • Gossypium

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Rostaniha
Volume 21, Issue 2 - Serial Number 60
December 2020
Pages 264-277

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History

  • Receive Date: 13 July 2020
  • Revise Date: 17 November 2020
  • Accept Date: 25 November 2020

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