Biological investigation of Lilium ledebourii in Iran

Document Type : Research Paper

Authors

1 MSc Student of Cellular and Developmental Biology, Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

2 Associate Prof., Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

3 PhD in Plant Physiology, Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

Abstract

Lilium ledebourii is a rare Asian species and is an endemic plant of Iran which grows in Gilan province in Damash highlands. In this study, the structural features of flower including the study of sections such as carpel, stamen, and tepals were performed. This plant had a full flower that consisted of six tepals (three petals and three petal-like sepals), six free stamens and a 3-lobed gynoecium. In the cross-section of tepals, the stomata as well as cells containing anthocyanins were observed. In stamens, anther (epidermis, endothecium, middle layers and an innermost tapetum), and filament were observed. In the gynoecium, ovary (ovary wall, locule, funiculus, anatropous ovule, different arrangements of vascular bundles, transmitting tissue and stomata), style (stylar canal and transmitting tissue), and dry stigma with elongated papillae tissue were observed. Besides, the presence of alkaloids in the bulb was tested using Wagner and Dragendorff reagents and the total alkaloid content in three types of methanol, ethanol, and butanol extraction was investigated. The ethanol extract had a significant difference with other extractions. The methanol extract was chosen for further investigation by gas chromatography-mass spectrometry (GC-MS) and two compounds of Pterin-6-carboxylic acid, and Pyrrolizin-1,7-dione-6-carboxylic acid, methyl (ester) were detected.

Keywords

Article Title [Persian]

بررسی زیست‌شناسی سوسن چلچراغ در ایران

Authors [Persian]

  • سلمان ملکی چره 1
  • فاطمه زرین کمر 2
  • مهرداد نظری 3
1 دانشجوی کارشناسی ارشد زیست‌شناسی سلولی و تکوینی، گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران
2 دانشیار گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران
3 دکترای فیزیولوژی گیاهی، گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران
Abstract [Persian]

سوسن چلچراغ (Lilium ledebourii) یک گونه نادر آسیایی از تیره سوسنیان است که بومی ایران بوده و در استان گیلان، در ارتفاعات منطقه داماش رشد می‌کند. در این مطالعه، ویژگی‌های ساختاری گل مشتمل بر مطالعه بخش‌هایی از قبیل برچه، پرچم و گلپارها انجام شد. این گیاه دارای یک گل کامل متشکل از شش گلپار (سه گلبرگ و سه کاسبرگ گلبرگ‌آسا)، شش پرچم آزاد و تخمدان سه برچه‌ای است. در سطح مقطع گلپارها، روزنه‌ها و همچنین سلول‌های حاوی آنتوسیانین‌ها مشاهده شدند. در پرچم‌ها، بساک (اپیدرم، لایه مکانیکی لایه‌های میانی و سلول‌های لایه پرستار میانی و میله مشاهده شد. در مادگی، تخمدان (دیواره، حفره، بند تخمک، تخمک آناتروپ، گروه‌های مختلف دسته‌های آوندی، بافت انتقال و روزنه‌ها)، خامه (کانال خامه و بافت انتقال دهنده) و کلاله خشک با بافت پاپیلای کشیده مشاهده شد. علاوه بر این، حضور آلکالوییدها در پیاز گل با استفاده از معرف‌های واگنر و دراژندورف مورد آزمایش قرار گرفت و محتوای کل آلکالوییدها در سه نوع عصاره متانولی، اتانولی و بوتانولی بررسی شد. عصاره اتانولی با سایر عصاره‌ها تفاوت معنی‌داری داشت. عصاره متانولی برای بررسی بیشتر با استفاده از کروماتوگرافی گازی-طیف‌سنجی جرمی
(GC-MS) انتخاب شد و دو ترکیب Pterin-6-carboxylic acid و Pyrrolizin-1,7-dione-6-carboxylic acid, methyl (ester) شناسایی گردیدند.

Keywords [Persian]

  • آلکالویید
  • تخمک آناتروپ
  • پاپیله
  • سوسنیان
  • کاسبرگ شبیه گلبرگ
  • گلپار

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Rostaniha
Volume 21, Issue 2 - Serial Number 60
December 2020
Pages 141-153

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History

  • Receive Date: 01 July 2020
  • Revise Date: 10 August 2020
  • Accept Date: 22 August 2020

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