Molecular detection of Lecanicillium fungicola on white button mushroom (Agaricus bisporus), using species-specific primer set

Document Type : Research Paper

Authors

1 Assistant Prof., Department of Science, Jundi-Shapur University of Technology, Dezful, P.O. Box 64615-334, Iran

2 Research Prof., Department of Botany, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, P.O. Box 1454-19395, Iran

3 Assistant Prof., Department of Biotechnology, Faculty of Agriculture, Ramin Agricultural and Natural Resources University, Ahwaz, P.O. Box 73637-63417, Iran

Abstract

The dry bubble disease (caused by Lecanicillium fungicola) is the most threatening disease of cultivated button mushrooms throughout the world. Most of the symptoms shown by the dry bubble are also shown by the wet bubble (Mycogone perniciosa) and they may even be confused. Early detection and precise monitoring of the diseases are very important for managing effective treatments. A PCR-based assay for a specific diagnosis of the disease in various stages of the disease development was developed. A primer pair designed based on ribosomal DNA generated two bands, 650 and 800 bp, specific for the detection of L. fungicola. Additionally, a simple and time-saving method for direct extraction of DNA from the affected sporophores is presented and the impact of sample pre-treatment on the efficiency of DNA isolation is highlighted. Here, we also report Simplicillium lamellicola from Iran, using the nuclear ribosomal internal transcribed spacer (ITS) region.
 

Keywords

Article Title [Persian]

شناسایی مولکولی بیماری حباب خشک قارچ دکمه‌ای با استفاده از آغازگرهای اختصاصی

Authors [Persian]

  • نادر رکنی 1
  • رسول زارع 2
  • علیرضا شافعی‌نیا 3
1 استادیار دانشکده علوم، دانشگاه صنعتی جندی شاپور، دزفول، ایران
2 استاد پژوهش، بخش تحقیقات رستنی‌ها، مؤسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران
3 استادیار گروه بیوتکنولوژی، دانشکده کشاورزی، دانشگاه کشاورزی و منابع طبیعی رامین، خوزستان، ایران
Abstract [Persian]

بیماری حباب خشک (ناشی از قارچ Lecanicilliumfungicola) یکی از مهلک‌ترین بیماری قارچ دکمه‌ای (Agaricus bisporus) در سراسر جهان است.  بسیاری از علایم این بیماری با یکی دیگر از جدی‌ترین بیماری‌های قارچ دکمه‌ای (بیماری حباب تر ناشی از Mycogone perniciosa) مشابه است. تشخیص سریع و به موقع  می‌تواند نقش تعیین‌کننده‌ای در مدیریت این بیماری ایفا نماید. برای تشخیص اختصاصی L. fingicola یک جفت آغازگر با توانایی تکثیر دو باند 650 و 800 جفت بازی از ناحیه  rDNA طراحی شد که در هر مرحله‌ای از توسعه بیماری قادر به تشخیص آن می‌باشد. در این تحقیق همچنین، روش ساده‌ای برای استخراج مستقیم دی.ان.آ. از کلاهک‌های قارچ دکمه‌ای ابداع شد و تاثیر پیش تیمار بافت کلاهک بر کارایی این روش مورد بررسی قرار گرفت. به علاوه، گونه Simplicillium lamellicola  با استفاده از آغازگرهای عمومی ناحیه آی.تی.اس. برای قارچ‌ها از ایران گزارش شده است.

Keywords [Persian]

  • استخراج دی.ان.آ
  • بیماری حباب تر
  • حباب خشک
  • نشانگر مولکولی
  • واکنش زنجیره‌ای پلیمراز

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Rostaniha
Volume 21, Issue 2 - Serial Number 60
December 2020
Pages 206-217

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History

  • Receive Date: 13 June 2020
  • Revise Date: 06 October 2020
  • Accept Date: 24 October 2020

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