Molecular and morphological evidence for two Clonostachys and one Sesquicillium species new to Iran

Document Type : Research Paper

Authors

1 MSc Student in Plant Pathology, Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Post Doc. Researcher, Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

3 Associate Prof., Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

4 Prof., Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

10.22092/bot.j.iran.2025.370380.1429

Abstract

To identify some fungal species associated with root–knot nematode (Meloidogyne species) or inhabiting the rhizosphere of Kiwifruit trees (Actinidia chinensis), infected roots and rhizospheric soil samples were collected from Kiwifruit orchards in Guilan Province (North of Iran). For fungal isolation from soil, one g of each soil sample was suspended in 100 mL of sterile distilled water. After making serial dilutions, 100 µL of each dilution was spread onto PDA-Rose Bengal agar supplemented with streptomycin. For isolation from nematodes, egg masses were surface-sterilized in 0.5% sodium hypochlorite, transferred to the same culture medium, and incubated at 25 °C in the dark for 10 days. The isolates were characterized using the morphological assessment and DNA sequence analyses of the internal transcribed spacer (ITS), translation elongation factor 1-α (tef1), and beta-tubulin (tub2) regions. Some isolates produced monomorphic or dimorphic conidiophores typical of members of the Clonostachys and Sesquicillium genera. Morphological and molecular data confirmed that, the isolates represented three species, including Clonostachys chloroleuca (from rhizospheric soli), C. rogersoniana (from a Meloidogyne sp. egg sac), and Sesquicillium aff. essexcoheniae (from rhizospheric soil). To the best of the authors’ knowledge, this is the first report of these species from Iran.

Keywords

Main Subjects

Article Title [Persian]

شواهد مولکولی و ریخت‌شناسی برای دو گونه Clonostachys و یک گونه Sesquicillium جدید در ایران

Authors [Persian]

  • مائده پورشیرمحمدی 1
  • محمدجواد پورمقدم 2
  • سالار جمالی 3
  • سیداکبر خداپرست 4
  • صدیقه موسی‌نژاد 3
1 دانشجوی کارشناسی ارشد گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
2 محقق پسادکتری گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
3 دانشیار گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
4 استاد گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
Abstract [Persian]

به منظور شناسایی برخی گونه‌های قارچی مرتبط با گونه‌های Meloidogyne یا موجود در ریزوسفر درختان کیوی (Actinidia chinensis)، نمونه‌هایی از گونه‌های Meloidogyne و خاک ریزوسفر از باغ‌های کیوی در استان گیلان جمع‌آوری شد. برای جداسازی قارچ از خاک، یک گرم از هر نمونه خاک در ۱۰۰ میلی‌لیتر آب مقطر سترون معلق گردید. پس از تهیه رقت‌های متوالی، ۱۰۰ میکرولیتر از هر رقت روی محیط کشت PDA-Rose Bengal حاوی استرپتومایسین پخش شد. برای جداسازی از نماتدها، توده‌های تخم با محلول هیپوکلریت سدیم نیم درصد ضدعفونی سطحی شده، به محیط کشت مشابه منتقل و به مدت ۱۰ روز در دمای ۲۵ درجه سلسیوس در تاریکی نگهداری شدند. جدایه‌ها با استفاده از ارزیابی ریخت‌شناختی و تجزیه و تحلیل توالی DNA نواحی فاصله‌دهنده رونویسی‌شونده داخلی (ITS)، فاکتور طویل شدن ترجمه یک آلفا (tef1) و بتا-توبولین (tub2) شناسایی شدند. برخی از جدایه‌ها کنیدیوفورهای مونومورفیک یا دیمورفیک معمول اعضای جنس‌های Clonostachys و Sesquicillium تولید کردند. داده‌های ریخت‌شناختی و مولکولی تأیید کردند که جدایه‌ها متعلق به سه گونه: Clonostachys chloroleuca (از ریزوسفر)، C. rogersoniana (از کیسه تخم .Meloidogyne sp) و Sesquicillium aff. essexcoheniae (از ریزوسفر) هستند. براساس اطلاعات نگارندگان، این نخستین گزارش از گونه‌های مذکور از ایران است.
 

Keywords [Persian]

  • تبارشناسی
  • تنوع زیستی
  • کیوی
  • نماتد مولد غده
  • Bionectriaceae

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History

  • Receive Date: 16 August 2025
  • Revise Date: 10 September 2025
  • Accept Date: 13 September 2025
  • Publish Date: 05 October 2025

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