Phylogenetic study of Adonis in Iran using nuclear and plastid DNA data

Document Type : Research Paper

Authors

1 Associate Prof., Department of Plant Sciences, Faculty of Biological Science, Alzahra University, Tehran, Iran

2 Prof., Department of Biology Medicinal Plants & Drugs Research Institute, Shahid Beheshti University, Tehran, Iran

3 PhD Student of Pharmacology, Department of Pharmacy, University of Oslo, Norway

10.22092/bot.j.iran.2025.370440.1432

Abstract

The genus Adonis is predominantly distributed in the temperate regions of the Northern Hemisphere. The present study is aimed to elucidate the phylogenetic relationship within the Iranian species of the genus Adonis. A molecular phylogenetic approach, based on nrDNA ITS and cpDNA trnL-F sequences, was applied to six species, one subspecies, and three varieties. Phylogenetic analyses were conducted using parsimony and Bayesian inference methods implemented in MEGA and PAUP softwares. The results confirmed the monophyly of Adonis sect. Adonis, consistent with previous studies. In the phylogenetic tree inferred from nrDNA ITS data, species of Trollius clustered together with the perennial species of Adonis (sect. Consiligo) in a single clade, whereas seven taxa of Adonis (sect. Adonis) formed a separate clade. In contrast, the cpDNA trnL-F data revealed that, all the species of Adonis (sect. Adonis and sect. Consiligo) grouped together in a single clade, while the perennial species of Trollius formed a distinct clade. In this analysis, the two clades were separated with 97% bootstrap support. Furthermore, the taxonomic position of A. globosa as a subspecies of A. microcarpa and A. microcarpa as a distinct species was confirmed.

Keywords

Main Subjects

Article Title [Persian]

مطالعه‌ تبارزایی جنس Adonis در ایران با بهره‌گیری از داده‌های DNA هسته‌ای و پلاستیدی

Authors [Persian]

  • منیژه پاکروان 1
  • علی سنبلی 2
  • الهام شجاعی‌نیا 3
1 دانشیار گروه علوم گیاهی، دانشکده علوم‌زیستی دانشگاه الزهراء، تهران، ایران
2 استاد گروه بیولوژی، پژوهشکده گیاهان و مواد اولیه دارویی، دانشگاه شهید بهشتی، تهران، ایران
3 دانشجوی دکترای داروسازی، گروه داروسازی، دانشگاه اسلو، نروژ
Abstract [Persian]

جنس چشم‌خروس (.Adonis L) از آلاله‌ئیان عمدتا در نواحی معتدل نیمکره‌شمالی پراکنش دارد. هدف از پژوهش حاضر روشن‌سازی روابط تبارزایی درون‌جنس Adonis در ایران بود. به این منظور، از رویکرد تبارزایی مولکولی بر پایه توالی‌های nrDNA ITS  و cpDNA trnL-F  در شش گونه، یک زیرگونه و سه واریته استفاده شد. تحلیل‌های تبارزایی با بهره‌گیری از روش‌های پارسیمونی و آنالیز بیزین در نرم‌افزارهای MEGA و PAUP انجام گرفت. نتایج تک‌تبار بودن بخش Adonis تأیید کرد که با مطالعات پیشین نیز هم‌خوانی دارد. در درخت تبارزایی حاصل ‌از داده‌های nrDNA ITS، گونه‌های Trollius به همراه گونه‌های چندساله Adonis  (بخش .Consiligo DC) در یک کلاد قرار گرفتند، درحالی‌که هفت آرایه از Adonis  (بخش Adonis) کلاد مجزایی را تشکیل دادند. در مقابل، داده‌هایcpDNA trnL-F  نشان داد که همه گونه‌های Adonis  (هر دو بخش Adonis  و Consiligo) در یک کلاد جای گرفتند، درحالی‌که گونه‌های چندساله Trollius کلاد مستقل و متمایزی را تشکیل دادند. در این تحلیل، جدایی دو کلاد با بوت‌استرپ 97 درصد پشتیبانی شد. افزون بر این، جایگاه رده‌بندی A. globosa C. Steinb. ex Rech. f.  به ‌عنوان یک زیرگونه و .A. microcarpa DC به ‌عنوان یک گونه مستقل مورد تأیید قرار گرفت.

Keywords [Persian]

  • تحلیل بیزین
  • تحلیل پارسیمونی
  • تنوع توالی
  • دی.ان.آ. ریبوروم هسته‌ای
  • سیستماتیک مولکولی
  • Ranunculaceae

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History

  • Receive Date: 17 August 2025
  • Revise Date: 01 September 2025
  • Accept Date: 06 September 2025
  • Publish Date: 23 September 2025

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