Foliar and pollen grain micromorphology of some species of Astragalus

Document Type : Research Paper

Authors

1 MSc Graduate, Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

2 Research Assistant Prof., Department of Natural Resources, Isfahan Agricultural and Natural Resources Research Center, Agricultural Research, Education and Extension Organization (AREEO), Isfahan, Iran

3 Prof., Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran

10.22092/bot.j.iran.2023.363687.1374

Abstract

The present study is focused on identifying the differences between the closely related sections Microphysa and Campylanthus in the genus Astragalus using micromorphological characteristics. For this purpose, the morphological characteristics of leaf epidermal cells and stomata as well as pollen grains have been studied. The results obtained from light- and scanning electron microscopy (LM and SEM) indicated that, stomatal type was typically anemocytic in most species, while the anisocytic type was only found in two species, viz. A. callistachys and A. microphysa. The epidermal cells in most species were observed to have a polygonal shape with straight anticlinal walls. However, in A. cephalanthus, both polygonal and irregular cell shapes as well as sinuate anticlinal walls were observed. All pollen grains shed as monad and exhibited small size, single shape, tricolporate aperture type, isopolarity and symmetry, with rounded or elongated pores. It was concluded that, the micromorphological data alone were not enough to separate these two sections and determine the limits of the species.

Keywords

Main Subjects

Article Title [Persian]

ریزریخت‌شناسی برگ و دانه گرده برخی گونه‌های Astragalus

Authors [Persian]

  • صدیقه اسکندری 1
  • آزاده اخوان روفیگر 2
  • شاهرخ کاظم‌پور اصالو 3
1 دانش‌آموخته کارشناسی ارشد گروه علوم گیاهی، دانشکده علوم‌ زیستی، دانشگاه تربیت مدرس، تهران، ایران
2 استادیار پژوهش بخش تحقیقات منابع طبیعی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان اصفهان، سازمان تحقیقات، آموزش و ترویج کشاورزی، اصفهان، ایران
3 استاد گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران
Abstract [Persian]

مطالعه حاضر بر شناسایی تفاوت‌های بین بخش‌های به هم نزدیک Microphysa و Campylanthus در جنس Astragalus (باقلاییان) با استفاده از ویژگی‌های ریزریخت‌شناسی متمرکز است. به این منظور، ریخت‌شناسی سلول‌های اپیدرمی و روزنه‌های برگ و دانه‌های گرده در هفت گونه بررسی شد. نتایج حاصل از مطالعات میکروسکوپی (نوری و الکترونی) نشان داد که نوع روزنه در بیشتر گونه‌ها به طور مشخص آنموسیتیک بود، در حالی که روزنه آنیزوسیتیک تنها در دو گونه A. callistachys و A. microphysa یافت شد. سلول‌های اپیدرمی در بیشتر گونه‌ها به شکل چندضلعی منظم با دیواره صاف مشاهده شدند، اما در گونه A. cephalanthus سلول‌های اپیدرمی به هر دو شکل چندضلعی و نامنظم و همچنین دیواره عمودی به شکل سینوسی دیده شد. براساس یافته‌های این مطالعه، پراکنش تمام دانه‌های گرده به طور منفرد، دارای اندازه کوچک و سه شیار منفذدار، جورقطب و متقارن با منافذ گرد یا کشیده بودند. طبق نتایج به دست آمده، داده‌های ریزریخت‌شناسی به تنهایی برای جداسازی این دو بخش و تعیین حدود گونه‌ها، معیار کافی تشخیص داده نشدند.

Keywords [Persian]

  • باقلاییان
  • دانه گرده
  • سلول‌های اپیدرمی
  • Campylanthus
  • Microphysa

References

Bagheri, A., Akhavan Roofigar, A., Abbasi, S., Maassoumi, A.A., Rutten, T. & Blattner, F.R. 2019. Pollen morphology of Astragalus section Hymenostegis (Fabaceae) and evaluation of its systematic implications. Grana 58(5): 328–336.
Bagheri, A., Maassoumi, A.A., Rahiminejad, M.R., Brassac, J. & Blattner, F.R. 2017. Molecular phylogeny and divergence times of Astragalus section Hymenostegis: An analysis of a rapidly diversifying species group in Fabaceae. Scientific Reports 7(1): 14033.
Bagheri, A., Rahiminejad, M.R. & Maassoumi, A.A. 2014. A new species of the genus Astragalus (Leguminosae-Papilionoideae) from Iran. Phytotaxa 178(1): 38–42.
Bahadur, S., Taj, S., Ahmad, M., Zafar, M., Gul, S., Shuaib, M., Butt, M.A., Hanif, U., Nizamani, M.M., Hussain, F. & Romman, M. 2022. Authentication of the therapeutic Lamiaceae taxa by using pollen traits observed under scanning electron microscopy. Microscopy Research and Technique 85(6): 2026–2044.
Buyukkartal, H.N., Çolgeçen, H., Pinar, N.M. & Erdoğan, N. 2012. Seed coat ultrastructure of hard-seeded and soft-seeded varieties of Vicia sativa. Turkish Journal of Botany 37(2): 270–275.
Castillon, E.E., Quintallina, J.A.V. & Salinas, A.D. 2020. Morphological range changes in Mexican Astragalus radicans Hornem. (Fabaceae: Galegeae): review of its taxonomy and nomenclature. Phytotaxa 470(2): 123–132.
Ceter, T., Ekici, M., Pinar, N.M. & Ozbek, F. 2013. Pollen morphology of Astragalus L. section Hololeuce Bunge (Fabaceae) in Turkey. Acta Botanica Gallica 160(1): 43–52.
Ekici, M., Yuzbas ̧, D. & Aytaç, Z. 2005. Morphology, pollen, seed structure and karyological study on Astragalus ovalis Boiss. and Balansa (sect. Ammodendron) in Turkey. International Journal of Botany 1(1): 74–78.
Ferguson, I.K. & Skvarla, J. 1981. The pollen morphology of the subfamily Papilionoideae (Leguminosae). Pp. 859–896. In: Polhill, R.M. & Raven, P.H. (eds), Advances in Legume Systematics. Royal Botanic Garden, Kew.
Ghahremaninejad, F. 2004. Value of trichome characteristics for separating of bifurcating Astragalus (Fabaceae) on sectional level. Turkish Journal of Botany 28(1/2): 241–245.
Ghahremaninejad, F., Joharchi, M.R, Fereidounfar, S. & Hoseini, E. 2016. Astragalus orientopersicus, sp. nov. (Fabaceae), a new taxon from the Khorassan province (Iran). Adansonia 38(1): 29–33.
Ghahremaninejad, F., Joharchi, M.R. & Memariani, F. 2022. Astragalus durandianus (sect. Trachycercis) as an Iranian endemic species: emended description, distribution, and conservation. Phytotaxa 550(1): 32–44.
Kazempour Osaloo, S., Maassoumi, A.A. & Murakami, N. 2003. Molecular systematics of the genus Astragalus L. (Fabaceae): Phylogenetic analyses of nuclear ribosomal DNA internal transcribed spacers and chloroplast gene ndh F sequences. Plant Systematics and Evolution 242(4): 1–32.
Kazempour Osaloo, S., Maassoumi, A.A. & Murakami, N. 2005. Molecular systematics of the Old World Astragalus (Fabaceae) as inferred from nrDNA ITS sequence data. Brittonia 57(4): 367–381.
Khan, A., Ahmad, M., Zafar, S., Abbas, Q., Arfan, M., Zafar, Sultana, S., Ullah, S.A., Khan, S., Akhtar, A., Kilic, O. & Ozdemir, F.A. 2022. Light and scanning electron microscopic observation of palynological characteristics in spineless Astragalus L. (Fabaceae) and its taxonomic significance. Microscopy Research and Technique 85(7): 2409–2427.
Khan, S., Jan, G., Ahmad, M., Gul, F., Zafar, M., Mangi, J. Din, Bibi, H., Sultana, S., Usma, A. & Majeed, S. 2021. Morpho-palynological assessment of some species of family Asteraceae and Lamiaceae of District Bannu, Pakistan on the bases of light microscope & scanning electron microscopy. Microscopy Research and Technique 84(6): 1220–1232.
Lashin, G.M. 2006. Comparative morphology of pollen grains of some taxa of tribe Trifolieae (Fabaceae: Papilionoideae) from Egypt. International Journal of Botany 2(3): 270–277.
Maassoumi, A.A. 2005. The Genus Astragalus in Iran, Vol. 5. Research Institute of Forests and Rangelands, Tehran (In Persian).
Maassoumi, A.A. 2020. The comprehensive online resource for Mega genus of Astragalus. Ver. 1 (Published in the Internet).
Mahmoodi, M., Ghahremaninejad, F. & Maassoumi, A.A. 2022. Diversity of vascular plants in Damirli Mountains (Zanjan Province, NW of Iran). Rostaniha 23(Suppl. 1): 1–131 (In Persian).
Majeed, S., Zafar, M., Ahmad, M., Kilic, O., Sultana, S., Raza, J., Yaseen, G., Gul, H., Mir, S., Lubna & Jabeen, M. 2020. Pollen morphological investigations of family Cactaceae and its taxonomic implication light microscopy and scanning electron microscopy. Microscopy Research and Technique 83(7): 767–777.
Mirzaei, L., Assadi, M., Nejadsatari, T. & Mehregan, I. 2015. Comparative seed and leaf micro-morphology of Colutea species (Fabaceae) from Iran. Environmental and Experimental Biology 13(4): 183–187.
Nabila, Ahmad, M., Zafar, M., Bahadur, S., Sultana, S., Taj, S., Celep, F., Majeed, S. & Rozina, 2022. Palynomorphological diversity among the Asteraceous honeybee flora: An aid to the correct taxonomic identification using multiple microscopic techniques. Microscopy Research and Technique 85(2): 570–590.
Noroozi, J., Talebi, A., Doostmohammadi, M. & Bagheri, A. 2020. The Zagros Mountain Range. Plant Biogeography and Vegetation of High Mountains of Central and South-West Asia. Pp. 185–214.
Oskouian, R., Kazempour Osaloo, S. & Maassoumi, A.A. 2007. Pollen morphology of Astragalus section Malacothrix and its allies (Fabaceae) in Iran. Journal of Science (University of Tehran) 32(4): 177–181.
Osman, A., Al-Ghamdi, F. & Guetat, A. 2014. Pollen morphology of some species of genus Astragalus L. (Fabaceae) in Northern region of Saudi Arabia. Life Science Journal 11(11): 1006–1019.
Ozbek, F., Ekici, M., Buyukkartal, H. & Pinar, N. 2021. Anatomy, palynology and micromorphology of the genus Astragalus L. (Fabaceae) section Uliginosi Gray in Turkey. Journal of the Institute of Science and Technology 11(4): 2525–2536.
Perveen, A. & Qaiser, M. 1998. Pollen flora of Pakistan-VIII Leguminosae (subfamily: Papilionoideae). Turkish Journal of Botany 22(2): 73–92.
Pinar, M., Ekici, M., Aytac, Z., Akan, H., Ceter, T. & Alan, S. 2009. Pollen morphology of Astragalus L. sect. Onobrychoidei DC. (Fabaceae) in Turkey. Turkish Journal of Botany 33(4): 291–303.
Podlech, D. & Zarre, Sh. (with collaboration of Ekici, M., Maassoumi, A.A. & Sytin, A.) 2013. A Taxonomic Revision of the Genus Astragalus L. (Leguminosae) in the Old World. Vols 1–3. Naturhistorisches Museum, Wien.
Ranjbar, M., Assadi, A. & Karamian, R. 2012. Notes on Astragalus sect. Macrophyllium with a Cytogenetic Report on its two Tetraploid Species. Journal of Science (Tarbiat Moallem University) 11(2): 211–226.
Raza, J., Ahmad, M., Zafar, M., Athar, M., Sultana, S., Majeed, S., Yaseen, G., Imran, M., Nazish, M. & Hussain, A. 2020. Comparative foliar anatomical and pollen morphological studies of Acanthaceae using light microscope and scanning electron microscope for effective microteaching in community. Microscopy Research and Technique 83(9): 1103–1117.
Ullah, F., Zafar, M., Ahmad, M., Dilbar, S., Shah, N., Sohail, A., Zaman, W., Iqbal, M., Bahadur, S. & Tariq, A. 2018. Pollen morphology of subfamily Caryophylloideae (Caryophyllaceae) and its taxonomic significance. Microscopy Research and Technique 81(7): 704–715.
Umber, F., Zafar, M., Ullah, R., Bari, A., Khan, M.Y., Ahmad, M. & Sultana, S. 2022. Copy for pollen morphology of selected taxa of family Asteraceae and Brassicaceae. Microscopy Research and Technique 85(1): 373–384.
Uzun, A., Aytac, Z. & Tulucu, F. 2021. Astragalus nurhakdagensis (sect. Hololeuce Bunge /Fabaceae), a new species from Turkey. Turkish Journal of Botany 45(6): 573–586.
Zarre, S. 2003. Hair micromorphology and its phylogenetic application in thorny species of Astragalus (Fabaceae). Botanical Journal of the Linnean Society 143(3): 323–330.
Zarre, S. & Azani, N. 2013. Perspectives in taxonomy and phylogeny of the genus Astragalus (Fabaceae): a review. Progress in Biological Sciences 3(1): 1–6.
Rostaniha
Volume 24, Issue 2 - Serial Number 69
December 2023
Pages 151-166

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History

  • Receive Date: 06 October 2023
  • Revise Date: 13 November 2023
  • Accept Date: 15 November 2023

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