Genetic and chemical diversity among Salvia multicaulis populations employing AFLP markers and the essential oil profile

Document Type : Research Paper

Authors

1 Department of Biology, Yadegar -e- Imam Khomeini (RAH) Shahre-rey Branch, Islamic Azad University, Tehran, Iran

2 MSc Postgraduate, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

10.22092/bot.j.iran.2023.362816.1363

Abstract

Salvia multicaulis is a widespread medicinal plant growing in north, northwest, and center of Iran. In this study, the aerial parts of 29 specimens belonging to five geographical populations were collected and dried at full flowering stage. AFLP molecular markers were applied for genetic diversity analysis. Essential oil was extracted by hydro-distillation and its chemical compositions were analyzed by GC/MS. AFLP results led to the recognition of two different genotypes. The results demonstrated high molecular affinity among Azerbaijan and Kurdistan populations. However, Tehran showed much more correlations with Isfahan and Kohgiluyeh-o-Boyerahmad populations. The AMOVA test results, demonstrated high within population genetic variability. STRUCTURE analysis revealed some degree of population genetic fragmentation that was principally due to genetic difference occurred between Azerbaijan and the rest of the studied populations. Overall, 40 constituents were determined among which, a-pinene (11.2–19.9%) and 1,8-cineole (10.2–20.4%) were distinguished as two major compounds. Tehran showed closer affinity to Isfahan and Kohgiluyeh-o-Boyerahmad. The results of molecular studies were in accordance with those obtained by the chemical compositions. Therefore, ecological factors and geographical distance as well as genetics play an important role in the species evolution and distribution in different parts of the country.

Keywords

Main Subjects

Article Title [Persian]

تنوع ژنتیکی و شیمیایی در جمعیت‌های Salvia multicaulis با استفاده از مارکرهای AFLP و ویژگی‌های اسانس

Authors [Persian]

  • گلاله مصطفوی 1
  • سهیلا حسامی 2
1 استادیار گروه زیست‌شناسی، واحد یادگار امام خمینی (ره) شهر ری، دانشگاه آزاد اسلامی، تهران، ایران
2 دانش‌آموخته کارشناسی ارشد، گروه زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
Abstract [Persian]

 
Salvia multicaulis (نعناییان) گیاهی دارویی با پراکنش وسیع در شمال، شمال‌غرب و مرکز ایران است. در این مطالعه، بخش‌های هوایی 29 نمونه مربوط به پنج جمعیت جغرافیایی جمع‌آوری و در مرحله گل‌دهی کامل خشک شدند. مارکرهای مولکولی AFLP برای آنالیز تنوع ژنتیکی به کار برده شد. اسانس به وسیله تقطیر آبی استخراج و ترکیبات شیمیایی آن توسط آنالیز GC/MS  شناسایی شدند. نتایج AFLP منجر به شناسایی دو ژنوتیپ متفاوت گردید. نتایج شباهت مولکولی بالایی بین جمعیت‌های آذربایجان و کردستان را نشان داد. با این حال، جمعیت تهران تشابه بیشتری به جمعیت‌های اصفهان و کهگیلویه و بویراحمد نشان داد. نتایج تست AMOVA تنوع بین جمعیتی بالایی را نشان داد. آنالیز STRUCTURE، درجاتی از قطعه قطعه شدن ژنتیکی جمعیت را آشکار ساخت که به طور کلی، به دلیل تفاوت ژنتیکی رخ داده بین جمعیت آذربایجان و سایر جمعیت‌های مورد مطالعه بود. در مجموع، 40 ترکیب شناسایی شد که در میان آن، آلفا-پینن (9/2-19/%11) و 1 و 8-سینئول (4/2-20/%10) به عنوان دو ترکیب اصلی تشخیص داده شدند. تهران بیشترین شباهت را به اصفهان و کهگیلویه و بویراحمد نشان داد. نتایج مطالعات مولکولی با نتایج به دست آمده از ترکیبات شیمیایی مطابقت داشت. بنابراین، فاکتورهای بوم‌شناختی و فاصله جغرافیایی و نیز ژنتیک نقش مهمی را در تکامل و انتشار گونه در نقاط مختلف کشور ایفا می‌کنند.

Keywords [Persian]

  • آنالیز GC/MS
  • پلی‌مورفیسم شیمیایی
  • تشابه مولکولی
  • مارکرهای مولکولی
  • نعناییان

References

Acree, T.E. & Arn, H. 2004. Flavornet and human odor space. Online at: http://www.flavornet.org/flavornet.html.
Adams, R.P. 2007. Identification of Essential Oil Components by Gas Chromatography/Mass Spectroscopy. Allured Publishing Corporation, Illinois. https://doi.org/10.1016/j.jasms.2005.07.008.
Amorati, R., Foti, M.C. & Valgimigli, L. 2013. Antioxidant activity of essential oils. Journal of Agricultural and Food Chemistry 61(46): 10835–10847. https://doi.org/10.1021/jf403496k.
Asadi-Samani, M., Khaledi, M., Khaledi, F., Samarghandian, S. & Gholipour A. 2019. Phytochemical properties and antibacterial effects of Salvia multicaulis Vahl., Euphorbia microsciadia Boiss., and Reseda lutea on Staphylococcus aureus and Acinetobacter baumanii. Jundishapur Journal of Natural Pharmaceutical Products 14(3): e63640. https://doi.org/10.5812/jjnpp.63640.
Clevenger, J. 1928. Apparatus for the determination of volatile oil. Journal of American Pharmacists Associations 17(4): 345–349. https://doi.org/10.1002/jps.3080170407.
Davis, P.H. 1982. Flora of Turkey and the Aegean Islands. Edinburgh University Press. Edinburgh. 948 pp. https://www.jstor.org/stable/10.3366/j.ctvxcrhxk.
Erasto, P. & Viljoen, A.M. 2008. Limonene a review: biosynthetic, ecological and pharmacological relevance. Natural Product Communications 3(7): 1193–1202. https://doi.org/10.1177/1934578X0800300728.
Fahed, L., Stien, D., Quaini, N., Eparvier, V. & Beyrouthy, M.E. 2016. Chemical diversity and antimicrobial activity of
Salvia multicaulis Vahl. essential oils. Chemical Biodiversity 13(5): 591–595. https://doi.org/10.1002/cbdv.201500332.
Fernández-Sestelo, M. & Carrillo, J.M. 2020. Environmental effects on yield and composition of essential oil in wild populations of spike lavender (Lavandula latifolia Medik.). Agriculture 10(12): 626.https://doi.org/10.3390/agriculture10120626.
Frankham, R., Ballou, J.D. & Briscoe, D.A. 2002. Introduction to Conservation Genetics. Cambridge: Cambridge University Press. https://doi.org/10.1017/CBO9780511808999.
Hamrick, J.L., Godt, M.J.W. 1990. Allozyme diversity in plant species. Pp. 43–63. In: AHD, B., Clegg, M.T., Kahler, A.L. & Weir, B.S. (eds), Plant Population Genetics, Breeding and Genetic Resources. Sinauer Associates.
Hedrick, P.W. 2005. A standardized genetic differentiation measure. Evolution 59(8): 1633–1638. https://doi.org/10.1111/j.0014-3820.2005.tb01814.x.
Jamzad, Z. 2012. Flora of Iran, No. 76, Lamiaceae. Research Institute of Forest and Rangelands, Tehran, Iran.
Janssen, P., Coopman, R., Huys, G., Swings, J., Bleeker, M., Vos, P., Zabeau, M. & Kersters, K. 1996.
Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy. Microbiology (Reading) 142(7): 1881–1893.
Jost, L. 2008. G(ST) and its relatives do no measure differentiation. Molecular Ecology 17(18): 4015–4026.
Kharazian, N. 2014. Chemotaxonomy and flavonoid diversity of Salvia L. (Lamiaceae) in Iran. Acta Botanica Brasillica 28(2): 281–92. http:/doi.org/10.1111/j.1365-294x.2008.03887.x.
Li, Z., Zou, J., Mao, K., Lin, K., Li, H., Liu, J., Källman, T. & Lascoux, M. 2012. Population genetic evidence for complex evolutionary histories of four high altitude juniper species in the Qinghai-Tibetan Plateau. Evolution 66(3): 831–845. http:/doi.org/10.1111/j.1558-5646.2011.01466.x.
Liu, J., Moller, M., Provan, J., Gao, L.M., Poudel, R.C. & Li, D.Z. 2013. Geological and ecological factors drive cryptic speciation of yews in a biodiversity hot spot. New Phytologist 199(4): 1093–1108. https://doi.org/10.1111/nph.12336.
Miguel, G., Simoes, M., Figueiredo, A.C., Barroso, J.G., Pedro, L.G. & Carvalho, L. 2004. Composition and antioxidant activities of the essential oils of Thymus caespititius, Thymus camphoratus and Thymus mastichina. Food Chemistry 86(2): 183–188. https://doi.org/10.1016/j.foodchem.2003.08.031.
Mohammadhosseini, M., Pazoki, A. & Akhlaghi, H. 2008. Chemical composition of the essential oils from flowers, stems, and roots of Salvia multicaulis growing wild in Iran. Chemistry of Natural Compounds 44(1): 127–128. https://doi.org/10.1007/s10600-008-0039-3.
Morteza-Semnani, K., Moshiri, K. & Akbarzadeh, M. 2005. The essential oil composition of Salvia multicaulis Vahl. Journal of Essential Oil-Bearing Plants 8(1): 6–10. https://doi.org/10.1080/0972060X.2005.10643412.
Peakall, R.O.D. & Smouse, P.E. 2006. Genalex 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6(1): 288–295. https://doi.org/10.1111/j.1471-8286.2005.01155.x.
Peakall, R.O.D. & Smouse, P.E. 2012. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 28(19): 2537–2539. http://doi.org/ 10.1093/bioinformatics/bts460.
Pritchard, J.K., Stephens, M., Donnelly, P. 2000. Inference of population structure using multilocus genotype data. Genetics 155(2): 945–959. https://doi.org/10.1093/genetics/155.2.945.
Qiao, Z., Xiao, D., Keovongkod, C., Wei K.H. & He, L.F. 2020. Assessment of the genetic diversity and population structure of Sophora tonkinensis in South China by AFLP markers, Biotechnology & Biotechnological Equipment 34(1): 975–985. https://doi.org/10.1080/13102818.2020.1812430.
Rustaiyan, A., Masoudi, S., Monfared, A. & Komeilizadeh, H. 1999. Volatile constituents of three Salvia species grown wild in Iran. Flavour and Fragrance Journal 14(5): 276–278. https://doi.org/10.1002/(SICI)1099-1026(199909/ 10)14:5<276::AID-FFJ825>3.0.CO;2-Y.
Sefidkon, F., Kalvandi, R., Atri, M. & Barazandeh, M. 2005. Essential oil variability of Thymus eriocalyx (Ronniger) Jalas. Flavour and Fragrance Journal 20(5): 521–524. https://doi.org/10.1002/ffj.1442.
Senatore, F., Apostolides Arnold N. & Piozzi, F. 2004. Chemical composition of the essential oil of Salvia multicaulis Vahl. var. simplicifolia Boiss. growing wild in Lebanon. Journal of Chromatography A 1052(1–2): 237–240. http://doi.org/10.1016/j.chroma.2004.08.095.
Setsuko, S., Ishida, K., Ueno, S., Tsumura, Y. & Tomaru, N. 2007. Population differentiation and gene flow within a metapopulation of a threatened tree, Magnolia stellata (Magnoliaceae). American Journal of Botany 94: 128–136. http://doi.org/10.3732/ajb.94.1.128.
Sheidai, M., Ziaee, S., Farahani, F., Talebi, S.M., Noormohammadi, Z. & Hasheminejad-Ahangarani Farahani, Y. 2014. Intra-specific genetic and morphological diversity in Linum album (Linaceae). Biologia 69(1): 32–39. https://doi.org/10.2478/s11756-013-0281-4.
Sheidai, M., Naji, M., Noormohammadi, M., Nouroozi M., Ghasemzadeh Baraki, S. 2016. Contemporary interspecific hybridization between Cirsium aduncum and C. Haussknechtii (Asteraceae): evidence from molecular and morpho-
logical data. Genetika 48(2): 497–514. http://doi.org/10.2298/GENSR1602497S.
Sunderland, M.A. & Hedge, I.C. 1982. Labiateae. Pp. 403–476. In: Rechinger, K.H. (ed.), Flora Iranica. Akademische Druckund Verlagsanstalt, Graz, Austria.
Talebi, S.M., Rezakhanlou, R. & Matsyura, A. 2017. Do we have infraspecific taxa of Salvia multicaulis Vahl. (Lamiaceae) in Iran? Ukrainian Journal of Ecology 7(4): 432–439. http://doi.org/10.15421/2017_128.
Talebi, S.M., Rezakhanlou, R. & Matsyura, A.V. 2019. Infraspecific genetic variation and population structure of Salvia nemorosa L. (Lamiaceae) in Iran. Ecologica Montenegrina 26: 127–136. https://doi.org/10.37828/em.2019.26.9.
Talebi, S.M., Askary, M., Khalili, N., Matsyura, A., Ghorbanpour, M. & Kariman, K. 2021. Genetic structure and essential oil composition in wild populations of Salvia multicaulis Vahl. Biochemical Systematics and Ecology 96: 104269–104275. https://doi.org/10.1016/j.bse.2021.104269.
Tavakoli, M., Soltani, S., Tarkesh Esfahani, M. & Karamian, R. 2022. Study on some environmental factor’s effects on Salvia multicaulis Vahl. essential oil composition in Hamadan province. Iranian Journal of Medicinal and Aromatic
Plants Research 38(4): 545–563 (In Persian). https://doi.org/10.22092/ijmapr.2022.358508.3162.
Tepe, B., Donmez, E., Unlu, M., Candan, F., Daferera, D., Vardar-Unlu, G., Polissiou, M. & Sokmen, A. 2004. Antimicrobial and antioxidative activities of the essential oils and methanol extracts of Salvia cryptantha (Montbret et Aucher ex Benth.) and Salvia multicaulis (Vahl.). Food Chemistry 84(4): 519–525. https://doi.org/10.1016/S0308-8146(03)00267-X.
Ulubelen, A., Topcu, G. & Johansson, C.B. 1997. Norditerpenoids and diterpenoids from Salvia multicaulis with antituberculous activity. Journal of Natural Products 60(12): 1275–1280. http://doi.org/10.1021/np9700681.
Vos, P., Hoger, R., Bleeker, M., Reijans, M., Van De Lee, T., Hornes, M., Frijteters, A., Pot, J., Peleman, J., Kuiper, M. & Zabeau, M. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23(21): 4407–4414. http://doi.org/10.1093/nar/23.21.4407.
Walker, J.B., Sytsma, K.J., Treutlein, J. & Wink, M. 2004. Salvia (Lamiaceae) is not monophyletic: implication for the systematics, radiation, and ecological specialization of Salvia and Tribe Mentheae. American Journal of Botany 91(7): 1115–1125. http://doi.org/10.3732/ajb.91.7.1115.
Wang, B., Zhang, Y., Chen, C.B., Li, XL., Chen, RY. & Chen, L. 2007 [Analysis on genetic diversity of different Salvia miltiorrhiza geographical populations in China]. Zhongguo Zhong Yao Za Zhi 32(19): 1988–1991. Chinese. PMID: 18161287.
Wang, S.Q. 2020. Genetic diversity and population structure of the endangered species Paeonia decomposita endemic to China and implications for its conservation. BMC Plant Biology 20: 510. http://doi.org/10.1186/s12870-020-02682-z.
Zhang, Y., Deng, K., Xie, L., Li, X., Wang, B. & Chen, L. 2009. Genetic diversity analysis and conservation of the Chinese herb Salvia miltiorrhiza collected from different geographic origins in China. African Journal of Biotechnology 8(19): 4849–4855.
 
Rostaniha
Volume 24, Issue 2 - Serial Number 69
December 2023
Pages 139-150

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History

  • Receive Date: 06 July 2023
  • Revise Date: 16 August 2023
  • Accept Date: 14 October 2023

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