First report of Circinella muscae for funga of Iran

Document Type : Systematics and Biodiversity of Fungi

Authors

1 PhD Student in Plant Pathology, Department of Plant Protection, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Associate Prof. in Mycology, Department of Plant Protection, Faculty of Agriculture, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

10.22092/bot.j.iran.2023.361307.1347

Abstract

During the study of fungal taxa in different regions of Markazi province (Iran) in spring of 2022, a fungal isolate with similar characteristics of Circinella Tiegh. & G. Le Monn. was obtained from the leaf spot symptom of Ficus altissima Blume. Based on the combined morphological characteristics and the sequence data of the ITS-nrDNA regions, recovered isolate (UTR29) was identified as C. muscae (Sorokin) Berl. & De Toni. Sporangiophores arising from the substrate or aerial mycelia, at first colorless then becoming pale brown, 6–11 μm in diam., erect, growing indefinitely, branching sympodial; branches sometimes 2–3- septate, at the end, bearing a single fertile sporangium, two fertile sporangia or a single fertile sporangium and a sterile spine; spines sterile, pale brown and cut off by a septum; sporangia diverse in size, multispored, globose to slightly dorsiventrally, with persistent wall, smooth, breaking, at first hyaline then becoming brownish yellow to gray with age, 30–61 × 29–58 μm in size; collumellae mostly hyaline, rarely light brown, diverse in shape, pyriform, oval, conical, subglobose, 18–30 × 15–28 μm in size; sporangiospores globose to oval, aseptate, grayish brown in color, black color in mass (inside of the sporangium), smooth, 3–7 × 3–6 μm in size. The nuclear ITS-rDNA of the isolate was amplified by ITS1/ITS4 primer pairs and the PCR product was sequenced. The resulting sequence was deposited in the GenBank (NCBI) with accession number of OQ550282. In the ML phylogenetic tree, isolate UTR29 was clustered with the other Circinella species with a bootstrap support of 100%. In addition, UTR29 was grouped with the other isolates of C. muscae (KF805762, KF805763 and MN431502) with a bootstrap support of 80%. Based on the bibliography, C. muscae is a new taxon for funga of Iran and Ficus altissima Blume is a new host (matrix nova) for C. circinans in the world. Living culture of isolate UTR29 was deposited in Agricultural Biotechnology Research Institute of Iran Culture Collection (Karaj, Iran) under accession number ABRIICC 10371.

Keywords

Article Title [Persian]

نخستین گزارش از Circinella muscae برای فونگای ایران

Authors [Persian]

  • عباس آتشی خلیل آباد 1
  • خلیل‌بِردی فتوحی فر 2
1 دانشجوی دکتری رشته بیماری شناسی گیاهی، گروه گیاه پزشکی، دانشکده کشاورزی، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران
2 دانشیار گرایش قارچ‌شناسی، گروه گیاه پزشکی، دانشکده کشاورزی، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران، ایران
Abstract [Persian]

طی مطالعه آرایه‌های قارچی در مناطق مختلف استان مرکزی در بهار 1401، جدایه قارچی با ویژگی‌های ریخت‌شناختی مشابه جنس Circinella از گیاه فیکوس (Ficus altissima) دارای علایم لکه‌برگی به دست آمد. براساس بررسی‌های ریخت‌شناختی از قبیل موج‌دار بودن یا نبودن اسپورانژیوفورها، حضور یا عدم حضور خارها روی انشعابات تولیدکننده اسپورانژیوم‌ها، تشکیل یا عدم تشکیل ساختارهای خاص در پایه خارها، ویژگی­‌های ظاهری ساختارهای خاص (منشعب یا بدون انشعاب و زگیل‌دار یا بدون زگیل)، مقاوم یا شکننده بودن اسپورانژیوم‌ها، شکل و اندازه اسپورانژیوسپورها، صاف یا زگیل‌دار بودن نوک ستونک‌ها و همچنین فیلوژنی مولکولی مبتنی بر توالی نوکلئوتیدی حاصل از تکثیر نواحی ITS-nrDNA، جدایه به دست آمده (UTR29) تحت گونه C. muscae شناسایی و توصیف گردید. براساس بررسی‌های انجام شده، گونه C. muscae آرایه جدیدی برای فونگای ایران بوده و برای نخستین بار گزارش و توصیف می‌شود. به علاوه، گیاه فیکوس میزبان جدیدی (matrix nova) برای C. muscae در دنیا معرفی می‌شود.

Keywords [Persian]

  • آرایه قارچی
  • تنوع زیستی
  • فیکوس
  • فیلوژنی
  • میزبان

References

Al-Bahry, S., Elshafie, A.E., Deadman, M., Al Sa'di, A., Al Raeesi, A. & Al Maqbali, Y. 2005. First report of Ganoderma colossum on Ficus altissima and Delonix regia in Oman. Plant Pathology 54(2): 245–245.
Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. 1990. Basic local alignment search tool. Journal of Molecular Biology 215: 403–410.
Banik, B., Debbarma, S., Majumdar, K. & Datta, B.K. 2020. A new distributional record of Ficus altissima Blume (Moraceae) in Tripura: an occasionally confused fig species with Ficus benghalensis L. Plant Science Today 7(4):
658–662.
Felsenstein, J. 1973. Maximum likelihood and minimum-steps methods for estimating evolutionary trees from data on discrete characters. Systematic Zoology 22: 240–249.
Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4): 783–791.
González, M.C., Murueta-Figueroa, N., Medina-Ortiz, C. & Hanlin, R.T. 2010. New record of Circinella muscae from a hydrocarbon polluted sand beach of Tabasco, Mexico. Mycotaxon 113: 111–117.
Hesseltine, C.W. & Fennell, D.I. 1955. The genus Circinella. Mycologia 47(2): 193–212.‏
Hwisa, N.T., Chandu, B.R., Katakam, P. & Nama, S. 2013. Pharmacognostical studies on the leaves of Ficus altissima blume. Journal of Applied Pharmaceutical Science 3(4): S56–S58.
Jamali, S. & Banihashemi, Z. 2012. Fungi associated with ascocarps of desert truffles from different parts of Iran. Journal of Crop Protection 1(1): 41–47.
Li, X., Xie, M., Yang, H., Chen, H., Yan, J., Wang, Z. & Fan, S. 2015. Pathogen identification and biological characteristics of leaf blight of Ficus altissima. Southwest China Journal of Agricultural Sciences 28(6): 2558–2562.
Nguyen, T.T. & Lee, H.B. 2018. Isolation and characterization of three Zygomycetous fungi in Korea: Backusella circina, Circinella muscae, and Mucor ramosissimus. Mycobiology 46(4): 317–327.
Refaei, J., Jones, E.B.G., Sakayaroj, J. & Santhanam, J. 2011. Endophytic fungi from Rafflesia cantleyi: species diversity and antimicrobial activity. Mycosphere 2(4): 429–447.
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. 2013. MEGA 6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30: 2725–2729.
‏Tieghem, P.É.L. van & Le Monnier, A.A.G. 1873. Recherches sur les Mucorinées. Annales des Sciences Naturelles, Botanique, 5e Série 17: 261–399.
Wang, H.K., Hyde, K.D., Soytong, K. & Lin, F.C. 2008. Fungal diversity on fallen leaves of Ficus in northern Thailand. Journal of Zhejiang University Science B 9: 835−841.
White, T.J., Bruns, T., Lee, S. & Taylor, J. 1990. Amplification & direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315–322. In: Inis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (eds), PCR Protocols: A Guide to Methods & Applications. Academic Press, San Diego, U.S.A.
Xia, G., Manawasinghe, I.S., Phillips, A.J., You, C., Jayawardena, R.S., Luo, M. & Hyde, K.D. 2022. Lasiodiplodia fici sp. nov., causing leaf spot on Ficus altissima in China. Pathogens 11(8): 840.
Zheng, R.Y., Liu, X.Y. & Wang, Y.N. 2017. Circinella (Mucorales, Mucoromycotina) from China. Mycotaxon 132(1): 43–62.‏
Zhong, S. & Steffenson, B.J. 2001. Virulence and molecular diversity in Cochliobolus sativus. Phytopathology 91(5): 469–476.

Files

History

  • Receive Date: 24 January 2023
  • Revise Date: 21 April 2023
  • Accept Date: 25 May 2023

Share

How to cite

Statistics