Molecular phylogeny of the genus Sanguisorba from Iran: Evidence based on cpDNA and nrDNA sequencing analysis

Document Type : Systematics and Biodiversity of Plants


1 Associate Prof., Department of Biology, Faculty of Science, University of Guilan, Rasht, 41938-33697, Iran

2 MSc Student, Department of Biology, Faculty of Science, University of Guilan, Rasht, 41938-33697, Iran

3 Assistant Prof., Department of Biology, Faculty of Science, Yasouj University, Yasouj, 74934-75918, Iran


In this research, the molecular phylogeny of the genus Sanguisorba including two species (S. officinalis and
S. minor), and the three subspecies (S. minor subsp. muricata, S. minor subsp. lasiocarpa, and S. minor subsp. minor) were studied from Iran using nrDNA ITS and cpDNA rpl32-trnL(UAG). For this purpose, 26 taxa, comprising four Iranian samples plus 22 previously sequenced data received from GenBank were analyzed. The phylogenetic relationships were reconstructed within Sanguisorba using maximum parsimony and Bayesian analyses. The results of nuclear sequence analysis showed separation of two subfamilies (Agrimoniinae and Sanguisorbinae), monophyly of Sanguisorba, complete separation of S. officinalis (in Sanguisorba clade) from S. minor and the three subspecies
(in Poterium clade). Although, the intraspecific relationship remained unresolved, but it was found that, the use of micro- and macromorphological criteria could be used as an important tool in different taxonomic ranks, especially in intraspecific identification. In addition, average sequence divergence, genetic differentiation, morphological, and micromorphological evidence are discussed.


Article Title [Persian]

مطالعه فیلوژنی جنس Sanguisorba در ایران: شواهدی مبتنی برتجزیه و تحلیل توالی DNA کلروپلاستی و هسته‌ای

Authors [Persian]

  • مرضیه بیگم فقیر 1
  • عادله دیلمی معزی 2
  • ربابه شاهی شاوون 3
1 دانشیار گروه زیست‌شناسی، دانشکده علوم، دانشگاه گیلان، رشت 33697-41938، ایران
2 دانشجوی کارشناسی ارشد، گروه زیست‌شناسی، دانشکده علوم، دانشگاه گیلان، رشت 33697-41938، ایران
3 استادیار گروه زیست‌شناسی، دانشکده علوم، دانشگاه یاسوج، یاسوج 75918-74934، ایران
Abstract [Persian]

در بررسی حاضر، فیلوژنی مولکولی جنس Sanguisorba متعلق به گل‌سرخیان (شامل دو گونه S. officinalis و S. minor) و سه زیرگونه (S. minor subsp. muricata, S. minor subsp. lasiocarpa و S. minor subsp. minor) از ایران با استفاده از nrDNA ITS و rpl32-trnL(UAG) مورد مطالعه قرار گرفت. به این منظور، 26 آرایه، شامل چهار نمونه از ایران به همراه 22 توالی از بانک ژن مورد آنالیز قرار گرفت. روابط فیلوژنتیکی گونه‌های Sanguisorba با استفاده از آنالیزهای ماکزیمم پارسیمونی و بایزین بازسازی شدند. نتایج آنالیز توالی هسته‌ای منجر به جدایی دو زیرطایفه (Agrimoniinae و Sanguisorbinae)، تک‌تباری جنس Sanguisorba و جدایی کامل
S. officinalis (روی شاخه Sanguisorba) از S. minor و سه زیرگونه آن (روی شاخه Poterium) شد. با وجودی که طی بررسی حاضر، روابط فروگونه به طور حل نشده باقی ماند، اما مشخص گردید که استفاده از معیارهای ریخت‌شناسی و ریزریخت‌شناسی می‌تواند
به عنوان ابزار مهمی برای تفکیک سطوح مختلف تاکسونومیک به ویژه، شناسایی فروگونه‌ایی استفاده شود. به علاوه، در این تحقیق، میانگین درصد واگرایی نوکلئوتیدی، تفاوت توالی‌ها، شواهد ریخت‌شناسی و ریزریخت‌شناسی مورد بحث قرار گرفته است.

Keywords [Persian]

  • بایزین
  • تاکسونومی
  • تعیین حدود
  • ماکزیمم پارسیمونی
  • واگرایی نوکلئوتیدها
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Volume 23, Issue 2 - Serial Number 67
December 2022
Pages 199-212
  • Receive Date: 27 July 2022
  • Revise Date: 07 September 2022
  • Accept Date: 13 September 2022
  • First Publish Date: 13 September 2022