A palynological study of the Lamiaceae in some habitats in Iran

Document Type : Systematics and Biodiversity of Plants

Authors

1 MSc Student, Department of Biology, Faculty of Sciences, Golestan University, Gorgan, Iran

2 Assistant Prof., Department of Biology, Faculty of Sciences, Golestan University, Gorgan, Iran

Abstract

Pollen grains of 33 species species belonging to 20 genera of the family Lamiaceae distributed in some habitats in Iran were studied using light and scanning electron microscope. For this purpose, pollen grains of Otostegia persica, Hymenocrater oxyodontus, and exine sculpture of three species, namely, Salvia splendens, S. hypoleuca, and Phlomis herba-venti subsp. lenkoranica were studied for the first time based on pollen morphology. The present study, therefore, showed considerable variation from very small, small, and medium to large size in pollen grains. In addition, the shapes varied from prolate-spheroidal, sub-prolate, sub-spheroidal, and sub-oblate to spheroidal in our study. The pollen grains colps observed into two different shapes i.e. tricolpate and hexacolpate, although octacolpate was observed in two species of Ziziphora clinopodioides and Z. tenuior. In the present study, 10 different exine ornamentation of pollen grains viz. micro-reticulate, reticulate, foveolate, foveolate-rugulose, foveolate-reticulate, bi-reticulate, rugolate-foveolate rugulose, regulate, and micro-foveolate-rugolate were also found. The present survey, therefore, indicateed that, pollen characters are valuable for taxonomic applications and may be a useful tool to differentiate variation between genera and species in the family Lamiaceae classification.
 
 

Keywords


Article Title [فارسی]

بررسی گرده‌شناسی نعناییان از برخی رویشگاه‌های ایران

Authors [فارسی]

  • زهرا حسنی 1
  • زهره کریمی 2
1 دانشجوی کارشناسی ارشد گروه زیست‌شناسی، دانشکده علوم، دانشگاه گلستان، گرگان، ایران
2 استادیار گروه زیست‌شناسی، دانشکده علوم، دانشگاه گلستان، گرگان، ایران
Abstract [فارسی]

دانه گرده 33 گونه متعلق به 20 جنس از نعناییان، پراکنده در برخی رویشگاه‌های ایران با استفاده از میکروسکوپ نوری و الکترونی روبشی  (SEM) مورد بررسی قرار گرفت. ریخت‌شناسی گرده گونه‌های Otostegia persica، Hymenocrater oxyodontus و مشخصات سطح اگزین در Salvia splendens وS. hypoleuca  و Phlomis herba-venti subsp. lenkoranica  برای نخستین بار مورد مطالعه قرار گرفت. این بررسی اندازه‌های متغیر قابل‌توجهی را در دانه‌های گرده به ‌صورت بسیار کوچک، کوچک، متوسط تا بزرگ نشان داد. به علاوه، در مطالعه حاضر، پنج نوع شکل دانه گرده به‌ صورت دوکی-گرد، نیمه‌دوکی، دوکی-کشیده، مخروطی-گرد و نیمه‌دوکی-پهن مشاهده شد. شیار در دانه‌های گرده اعضای این تیره به دو شکل سه‌‌شیاری و شش‌شیاری بود، گرچه به ‌صورت هشت‌‌شیاری نیز در گونه‌هایZiziphora clinopodioides  وZ. tenuior  مشاهده شد. ده نوع تزیینات مختلف اگزین نیز به ‌صورت مشبک (reticulate) و ریزمشبک (micro-reticulate)، چاله یا حفره‌دار (foveolate)، چین‌دار (rugulate)، چین ظریف (rugulose)، دوبار مشبک (bi-reticulate)، حفره‌دار-چین‌دار (rugolate-foveolate)، حفره‌دار-مشبک (foveolate-reticulate)، حفره‌دار-چین ظریف (foveolate-rugulose) و ریزحفره‌دار-چین‌دار (micro-foveolate-rugolate) مشاهده شد. این بررسی نشان داد که مشخصات دانه‌های گرده برای کاربردهای طبقه‌بندی ارزشمند بوده و ممکن است ابزار مفیدی برای تمایز بین جنس‌ها و گونه‌ها در طبقه‌بندی نعناییان باشد.
 
 

Keywords [فارسی]

  • اگزین
  • تزیینات
  • سه‌شیاری
  • شش‌شیاری
  • شکل دانه گرده
Abu-Asab, M.S. & Cantino, P.D. 1992. Pollen Morphology in Subfamily Lamioideae (Labiatae) and its Phylogenetic Implications. Pp. 97–122. In: R.M. Harley & T. Reynolds (eds), Advances in Labiatae Science. Royal Botanic Gardens, Kew, Richmond, Surrey, UK.
Abu-Asab, M.S. & Cantino, P.D. 1994. Systematic implications of pollen morphology in subfamilies Lamioideae & Pogostemonoideae (Labiatae). Annals of the Missouri Botanical Garden 81(4): 653–686.
Aktaş, K., Özdemirm, C., Özkanp, M. & Baran, P. 2020. Pollen morphology of some Turkish Salvia L. (Lamiaceae: Mentheae) species. Planta Daninha 38: 1–14. https://doi: 10.1590/s0100-83582020380100012.
Al-Taie, S.S.K. 2019. Palynological study in some medicinal species of Lamiaceae (Labiatae) family in Iraq. Plant Archives 19(1): 1003–1008.
Azimishad, F., Sheidai, M., Keshavarzi, M., Talebi, S. & Noormohammadi, Z. 2020. Systematic significance of micromorphological and palynological characteristics in Lagochilus Bunge ex Benth. (Lamiaceae) in Iran. Acta Biologica Szegediensis 63(2): 143–155. https://doi: 10.14232/abs.2019.2.143-155.
Azizian, D., Jamzad, Z. & Serpooshan, F. 2001. Pollen morphology & taxonomy of the genus Nepeta sect. Psilonepeta (Labiatae) in Iran. Iranian Journal of Botany 9(l): 19–26.
Azizian, D. & Moore, D.M. 1982. Morphological & palynological studies in Phlomis L., Eremostachys Bunge & Paraphlomis Prain (Labiatae). Botanical Journal of the Linnean Society 85: 225–248.
Badamtsetseg, B., Myoung, L.S. & Yuon, L.H. 2012. Pollen morphology of the family Lamiaceae
in Mongolia. Journal of Korean Nature 5(2): 169–179. https://doi: 10.7229/jkn.2012.5.2.169.
Briquet, J. 1897. Labiatae. Pp. 290–291. In: A. Engler & K. Prantl. (eds), Die natürlichen Planzenfamilien, Nachträge zum II.–IV. Teil. Leipzig: Engelmann.
Cantino, P.D. 1992. Evidence for a polyphyletic origin of the Labiatae. Annals of the Missouri Botanical Garden 79: 361–379.
Cantino, P.D. & Sander, R.W. 1986. Subfamilial classification of Labiatae. Systematic Botany 11: 163–185.
Cantino, P.D., Harley, R.M. & Wagstaff, S.J. 1992. Genera of Labiatae: Status and Classification. Pp. 511–522. In: R.M. Harley & T. Reynolds (eds), Advances in Labiatae Science. Royal Botanic Gardens, Kew, Richmond, Surrey, UK.
Celenk, S. Tamimcilar, G. Bicakci, A. Kaynak, G. & Malyer, H. 2008. A palynological study of the genus Mentha L. (Lamiaceae). Botanical Journal of the Linnean Society 157(1): 141–154. https://doi.org/10.1111/j.1095-8339.2008.00789.x
Doaigey, A.R., El-Zaidy, M.A., Alfarhan, A.E., Milagy, S. & Jacob, T. 2018. Pollen morphology of certain species of the family Lamiaceae in Saudi Arabia. Saudi Journal of Biological Science 25: 354–360.
Duarte, M. & Lopes, J.F. 2007. Stem and leaf anatomy of Plectranthus neochilus Schltr. Lamiaceae. Revista Brasileira de Farmacognosia-Brazilian Journal of Pharmacognosy 17(4): 549–556.
Erdtman, G. 1945: Pollen morphology and plant taxonomy IV. Labiatae, Verbenacae and Avicenniaceae. Svensk Botanisk Tidskrift 39: 279–285.
Erkara, I.P., Kose, Y.B. & Alan, S. 2011. Pollen morphology of some Turkish Ajuga L. (Lamiaceae) & its taxonomic value. Bangladesh Journal Botany 40: 29–33.
Firdous, S., Ahmed, H., Hussain, M. & Shah, M. 2015. Pollen Morphology of Ajuga L., Lamium L. and Phlomis L. (Lamiaceae) from District Abbottabad Pakistan. Pakistan Journal of Botany 47(1): 269–274.
Ghahremaninejad, F., Ataei, N. & Nejad Falatoury, A. 2017. Comparison of angiosperm flora of Afghanistan and Iran in accordance with APG IV system. Nova Biologica Reperta 4(1): 73–97 (In Persian with English abstract).
Gráfica, P. 2020. ‘Planta Daninha’. Pp. 1–14. https://doi: 10.1590/S0100-83582020380100012.
Harley, R.M., Atkins, S., Budantsev, A.L., Cantino, P.D., Conn, B.J., Grayer, R., Harley, M.M., Dekok, R., Krestovskaja, T., Morales, R., Paton, A.O. & Upson, T. 2004. Labiatae. Pp. 167–275. In: The Families and Genera of Vascular Plants, Flowering Plants: Dicotyledons (Lamiales except Acanthaceae including Avicenniaceae). J.W. Kadereit (ed.), Vol. VII. Berlin & Heidelberg. Springer Verlag.
Hasaninejad, M., Jamzad, Z. & Uosofi, M. 2011. A palynological study of Scutellaria L. (Lamiaceae) in Iran. Taxonomy & Biosystematics 3(7): 33–44.
Henderson, D.M., Prentice, H. & Hedge, I.C. 1968. Pollen morphology of Salvia and some related taxa. Grana Palynology 8: 70–85.
Huang, T.C. 1972. Pollen Flora of Taiwan. National Taiwan University, Botany Department Press. 297 pp.
Jafari, A. & Nikian, M. 2008. Micromorphological, anatomical and pollen ornamentation study on four desert species of Salvia in center of Iran. Asian Journal of Plant Sciences 7(8): 736–741.
Jamzad, Z. 2012. Lamaiceae. Flora of Iran. Research Institute of Forests & Rangelands, Tehran. Vol. 76. 1074 pp. (In Persian).
Jamzad, Z. 2013. A survey of Lamiaceae in the flora of Iran. Rostaniha 14(1): 59–67.
Kaya, A. & Kutluk, H. 2007. Pollen morphology of Acinos Miller species growing in Turkey. Journal of Integrative Plant Biology 49: 1386–1392.
Noroozi, M., Ghahremaninejad, F., Bogler, D.M., Witherspoon, J., Ryan, G.L., Miller, J.S., Riahi, M. & Cohen, J.I. 2022. Parsing a plethora of pollen: the role of pollen size and shape in the evolution of Boraginaceae. Cladistics. https://doi.org/10.1111/cla.12488.
Moon, H.K. 2008. Systematic Studies in Mentheae (Lamiaceae): Morphology, Evolutionary Aspects and Phylogeny. Unpublished, D.Phil. Thesis, K.U., Leuven.
Moon, H.K. & Hong S.P. 2003. Pollen morphology of the genus Lycopus (Lamiaceae). Annales Botanici Fennici 40: 191–198.
Moon, H.K., Vinckier, S., Smets, E. & Huysmans, S. 2008. Comparative pollen morphology and ultrastructure of Mentheae subtribe Nepetinae (Lamiaceae). Review of Palaeobotany and Palynology 149: 174–186.
Moore, P.D., Webb, J.A. & Collinson, M.E. 1991. Pollen Analysis. Oxford: Blackwell.
Myoung, L.S. & Yuon, L.H. 2012. Pollen morphology of the family Lamiaceae in Mongolia. Journal of Korean Nature 5(2): 169–179. https://doi: 10.7229/jkn.2012.5.2.169.
Naderifar, M., Sonboli, A. & Gholipour, A. 2015. Pollen morphology of Iranian Dracocephalum L. (Lamiaceae) and its taxonomic significance. Bangladesh Journal of Plant Taxonomy 22(2): 99.
Özler, H., Pehlivan, S., Celep, F., Dogan, M., Kahraman, A. & Yavru, F.A. 2013. Pollen morphology of Hymenosphace and Aethiops section of the genus Salvia L. (Lamiaceae) in Turkey. Turkish Journal of Botany 37: 1070–1084.
Pădure, I.M. 2014. ‘Nepeta (Lamiaceae) morphology and anatomy in Romania and their taxonomic significance’. Plant, fungal and habitat diversity investigation and conservation. Proceedings of IV BBC-Sofia 2006 (https://www.researchgate.net/publication/268215959). doi: 10.13140/2.1.2828.5129.
Punt, P., Hoen, P., Blackmore, S., Nilsson, S. & Thomas, A.L. 2007. Glossary of pollen and spore terminology. Review of
Palaeobotany and Palynology 143(1–2): 1–81. https://doi.org/10.1016/j.revpalbo.2006.06.008.
Ranjbar, M., Pakatchi, A., & Radjabian, T. 2015. Pollen morphology study of Salvia sect. Plethiosphace & sect. Hemisphace (Lamiaceae) in Iran. Nova Biologica Reporta 1(2): 16–28 (In Persian with English abstract).
Rasband, W.S. 1997–2021. ImageJ, National Institutes of Health, Bethesda, Maryl & USA. http://rsb.info.nih.gov/ij.
Razavi, S.M., Ghaffari, A.A. & Bidarlord, M. 2018. Pollen morphology of some Nepeta L. species in NW Iran. Botany Letters 165(2): 286–291. doi: 10.1080/23818107.2017.1411830.
Rechinger, K.H., Hedge. I.C. Ietswaart, J.H., Jalas, J., Mennema, J. & Seybold, S. 1982. Flora Iranica (Labiatae). Akademische Drucku. Verlagsanstalt. Graz, Akademische Verlagsgesellschaft. Salzburg 150: 403–476.
Salmaki, Y., Jamzad, Z., Zarre, S. & Bräuchler, C. 2007. Pollen morphology of Stachys (Lamiaceae) in Iran and its systematic implication. Flora 203: 627–639.
Sayyar, M. 2014. Morphology of Pollen Grains of the Genus Teucrium L. Belongs to the Family Lamiaceae in Ardabil Province, Payam-e Noor University Publication. Tehran, Iran (Dissertation, In Persian).
Stevens, P.F. 2012. "Lamiales (Lamiaceae Family)". Angiosperm Phylogeny Website. Retrieved 25 March 2015.
Talebi, S., Nahooji, M., Yarmohammadi, M. & Azizi, N. 2020. Pollen morphological traits analysis of eighteen ‘Nepeta’ species in Iran. Mediterranean Botany 41(1): 85–99. https://doi.org/10.5209/mbot.62919.
Thorne, R.F. 1992. Classification and geography of the flowering plants. The Botanical Review 58: 225–327.
Wagstaff, S.J. 1992. A Phylogenetic Interpretation of Pollen Morphology in the Tribe Mentheae (Labiatae). Pp. 113–124. In: R.M. Harley & T. Reynolds (eds), Advances in Labiatae Science. Royal Gardens, Kew, Richmond, Surrey. UK.
Zafar, M., Ahmad, M. & Khan, M.A. 2006. Pollen flora of Lamiaceae from Rawalpindi-Pakistan. Gomal University Journal Research 22(2): 1–5.
Zhao, F., Chen, Y.P., Salmaki, Y., Drew, B.T., Wilson, T.C., Scheen, A.C., Celep, F., Bräuchler, C., Bendiksby, M., Wang, Q. & Min, D.Z. 2021. An updated tribal classification of Lamiaceae based on plastome phylogenomics. BMC Biology 19(2):
1–27. https://doi.org/10.1186/s12915-020-00931-z.
Volume 23, Issue 1 - Serial Number 63
September 2022
Pages 25-44
  • Receive Date: 12 December 2021
  • Revise Date: 06 February 2022
  • Accept Date: 13 April 2022
  • First Publish Date: 13 April 2022