Study of morphological variation of Nostoc punensis and Desmonostoc salinum isolated from soils around lakes of Golestan province (N Iran) in different culture media

Document Type : Research Paper

Author

Assistant Prof., Department of Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

Studying the cyanobacteria strains grown on different culture media has led to controversies when they are identified by classic keys. Hence, the main target of this study is to identify cyanobacteria strains and compare their distribution in different soil, then identification the culture media with the highest stability and least effect on their variations. In this regard, soils samples of Ala gol, Aji gol, and Alma gol lakes belong to Golestan province (north of Iran), were collected and cyanobacteria taxa were isolated, and identified. In addition, an attempt was made to determine their incidence with some physicochemical characteristics of the soils. Morphological observation showed that five different strains were identified in Ajigol, four different strains in Alagol, and two different strains in Alma gol. Results of morphological identification showed that, Nostoc punensis and Anabaena sp. are common in Ala gol and Aji gol lakes, except Alma gol lake, where its EC was the highest and Dulcicalothrix alborzica and Neowestiellopsis persica were the only species found in the stands. Then two strains of Nostoc punensis and Desmonostoc salinum which had very variable morphology in culture media, were grown on Z8 and BG-11 culture media without nitrogen, and morphological variations of cells were measured from the inoculating time up to the end of the stationary phase of their growth. In addition, morphological variations showed that, vegetative cells and akinetes of both strains changed into spiral form and large and heterocytes also had a bony form on BG-11 medium but retained their natural form in Z8 medium. Accordingly, the Z8 medium is suggested to be used for its stability as preferred culture media to provide less morphological variations.
 
 

Keywords

Article Title [Persian]

مطالعه تنوع ریخت‌شناختی Nostoc punensis و Desmonostoc salinum جدا شده از خاک‌های اطراف دریاچه‌های استان گلستان

Author [Persian]

  • بهاره نوروزی
استادیار زیست‌شناسی مولکولی سیانوباکتری‌ها، گروه بیوتکنولوژی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران، ایران
Abstract [Persian]

در مطالعه سویه‌های سیانوباکتریایی، تنوع ریخت‌شناختی سلول‌ها در هنگام رشد در محیط‌های کشت مختلف اغلب منجر به شکل‌گیری اختلاف نظرهای بسیاری در هنگام شناسایی آرایه‌ها با استفاده از کلیدهای شناسایی کلاسیک می‌شود. از این رو، هدف اصلی این مطالعه شناسایی سویه‌های سیانوباکتریایی و مقایسه توزیع آن‌ها و سپس تعیین بهترین محیط کشت با بیشترین ثبات و کمترین تأثیر بر تغییرات ریخت‌شناختی آن‌ها است. در این راستا، نمونه‌هایی از خاک اطراف دریاچه‌های آلاگل، آجی‌گل و آلماگل (استان گلستان) جمع‌آوری و آرایه‌های سیانوباکتریایی موجود در آن‌ها جداسازی و شناسایی شدند. به علاوه، ارتباط حضور سویه‌های مختلف سیانوباکتریایی با ویژگی‌های فیزیکوشیمایی خاک نیز بررسی گردید. براساس نتایج به‌ دست آمده، در مجموع، پنج سویه از آجی‌گل، چهار سویه از آلاگل و دو سویه از آلماگل گزارش شد. همچنین، نتایج بررسی‌ها نشان داد که دو سویه Nostoc punensis و Anabaena sp. در آلاگل و آجی‌گل به ‌صورت مشترک و دو سویه Dulcicalothrix alborzica و Neowestiellopsis persica تنها در خاک منطقه آلماگل با بیشترین میزان EC حضور داشتند. سپس، دو سویه Nostoc punensis و Desmonostoc salinum که دارای بیشترین تغییرات ریخت‌شناسی در محیط کشت بودند، جهت ادامه مطالعات انتخاب شدند و این تغییرات در دو محیط کشتZ8  و BG-11 فاقد نیترات از زمان تلقیح تا پایان مرحله ایستایی رشد اندازه‌گیری گردید. نتایج حاصل از بررسی تغییرات ریخت‌شناختی نشان داد که سلول‌های رویشی و آکاینت هر دو سویه در محیط کشت BG-11 مارپیچ، بزرگ و هتروسیست‌ها استخوانی شکل شدند، اما شکل طبیعی خود را در محیط کشت Z8 حفظ کردند. بر این اساس، پیشنهاد می‌شود از محیط کشت Z8 به عنوان محیط کشت ترجیحی برای حفظ ثبات سلول‌ها و تغییرات ریخت‌شناختی کمتر استفاده شود.
 

Keywords [Persian]

  • آنالیز مورفومتریک
  • تنوع ریخت‌شناسی
  • سیانوباکتری‌های هتروسیست‌دار
  • محیط کشت Z8

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Rostaniha
Volume 22, Issue 2 - Serial Number 62
December 2021
Pages 297-309

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History

  • Receive Date: 11 December 2021
  • Revise Date: 31 December 2021
  • Accept Date: 17 January 2022

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