Molecular phylogenetic study of toxic cyanobacterium Anabaena sp. strain B3 isolated from Lavasan Lake, Tehran (Iran)

Document Type : Systematics and Biodiversity of Plants

Authors

1 Master student of Genetic, Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

2 Assistant Prof., Department of Biotechnology, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Assistant Prof., Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

Abstract

Lavasan Lake situated in the north-east of Tehran (Iran) is a complex ecosystem of cyanobacterial strains with an ability to produce a wide range of toxic natural compounds that may lead to many problems or even death of humans and animals. Based on the report of toxic cyanobacterial strains present in the Lavasan Lake, the morphological and phylogenetic study of the toxic strain purified from Lavasan Lake water, was performed using 16S rRNA and ITS gene markers. According to the results, the phylogenetic tree was drawn based on 16S rRNA gene, non-toxic strains with the toxic strain Anabaena sp. strain B3 (CCC B3) were not clustered within a cloud. In addition, the ability to produce toxins appears to be lost over time. The results of ITS gene analysis using Mfold showed that, the most important difference between the toxic strain Anabaena sp. B3 with other non-toxic strains of Anabaena sp. has the number of nucleotides at terminal bilateral bulge and the number of loops at bilateral bulge in the D1-D1 helix. However, Box B helix structure was not different in any of the strains. This is the first report of molecular phylogeny of a toxin-producing cyanobacterium isolated from the Lavasan Lake.
 
 

Keywords

Article Title [Persian]

مطالعه فیلوژنی مولکولی سویه سمی Anabaena sp. B3 جدا شده از دریاچه لواسان (تهران)

Authors [Persian]

  • مریم ریوندی 1
  • بهاره نوروزی 2
  • حسین فهمیی 3
1 دانشجوی کارشناسی ارشد ژنتیک، دانشکده علوم و تکنولوژی پیشرفته، واحد علوم پزشکی تهران، دانشگاه آزاداسلامی، تهران، ایران
2 استادیار زیست‌شناسی مولکولی سیانوباکتری‌ها، گروه بیوتکنولوژی، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران، ایران
3 استادیار گروه ژنتیک، دانشکده علوم و فناوری‌های نوین، دانشگاه علوم پزشکی آزاد اسلامی، تهران، ایران
Abstract [Persian]

دریاچه لواسان واقع در شمال‌شرق تهران، اکوسیستم پیچیده‌ای متشکل از سویه‌های سیانوباکتری‌ها با توانایی تولید طیف وسیعی از ترکیبات طبیعی سمی است که ممکن است منجر به بروز مشکلات فراوان و یا حتی مرگ انسان‌ها و حیوانات شوند. با توجه به گزارش حضور سویه‌های سمی سیانوباکتریایی در دریاچه لواسان، در این مطالعه، بررسی‌های ریخت‌شناختی و فیلوژنی سویه سمی خالص‌سازی شده از آب دریاچه مذکور، با استفاده از نشانگرهای ژنی 16S rRNA و آی.تی.اس. صورت گرفت. براساس نتایج به‌ دست آمده از درخت فیلوژنی رسم شده مبنی بر ژن 16S rRNA، سویه‌های غیرسمی با سویه سمی Anabaena sp. B3 (CCC B3)، درون یک کلاد خوشه‌بندی نشدند. به علاوه، به نظر می‌رسد که توانایی تولید سم در طول زمان از بین رفته است. نتایج حاصل از آنالیز ژن آی.تی.اس. با استفاده از Mfold نشان داد که مهم‌ترین تفاوت سویه سمی Anabaena sp. B3 با سایر سویه‌های غیرسمی Anabaena sp.، تعداد نوکلئوتیدهای لوپ انتهایی دو طرفه و تعداد لوپ‌های دو طرفه میانی در مارپیچ D1-D1 است. این در حالی است که ساختمان مارپیچ B Box در هیچ‌کدام از سویه‌ها تفاوتی نداشت. مطالعه حاضر، جزو نخستین تحقیقات انجام شده روی فیلوژنی مولکولی سیانوباکتریوم سمی در دریاچه لواسان است.
 
 

Keywords [Persian]

  • اکوسیستم
  • ژن‌های ساختاری
  • خوشه‌بندی
  • سیانوباکتری
  • 16S rRNA
  • Internal Transcribed Spacer (ITS)

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Rostaniha
Volume 22, Issue 1 - Serial Number 61
October 2021
Pages 120-133

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History

  • Receive Date: 20 July 2021
  • Revise Date: 12 August 2021
  • Accept Date: 18 August 2021

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