Isolation and identification of entomopathogenic fungi from cultivated and natural soils in Kermanshah province (West of Iran)

Document Type : Research Paper

Authors

1 MSc Student, Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran

2 Assistant Prof., Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran

Abstract

An investigation was carried out on the occurrence and distribution of entomopathogenic fungi in 41 soil samples collected from cultivated and natural regions in Kermanshah province (West of Iran) from July 2017 to April 2018. Among 41 soil samples, 114 fungal isolates were recovered with 39 from forests, 38 from fields, 22 from rangelands and 15 from garden soil. Based on the morphological characters and phylogeny of DNA sequence data for the internal transcribed spacer (ITS) rDNA and comparing the sequences with that available in NCBI database, the entomopathogenic fungi recovered were identified as: Aspergillus nomius*, Fusarium oxysporum, F. equiseti, Fusarium sp., Penicillim solitum, P. sizovae*, Penicillium sp., Alternaria chlamydosporigena, Meyerozyma guilliermondii, Paramyrothecium roridum, Chaetomium elatum, Beauveria bassiana*, B. pseudobassiana, and
B. tenella*. The asterisk species are the new records for the mycobiota of Iran.
 
 

Keywords

Article Title [Persian]

جداسازی و شناسایی قارچ‌های بیمارگر حشرات از خاک‌های زراعی و غیرزراعی در استان کرمانشاه

Authors [Persian]

  • هادی مهرمرادی 1
  • صمد جمالی 2
  • حمیدرضا پوریان 2
1 دانشجوی کارشناسی ارشد بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران
2 استادیار بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران
Abstract [Persian]

در راستای شناسایی قارچ‌های بیمارگر حشرات (انتوموپاتوژن‌ها)، از خاک‌های زراعی و غیرزراعی استان کرمانشاه نمونه‌‌برداری صورت گرفت. در مجموع، 41 نمونه خاک از عمق ۱۵-5 سانتی‌متری خاک از 14 شهرستان‌ استان کرمانشاه جمع‌آوری گردید. در نهایت، 114 جدایه قارچی از خاک‌های مختلف جداسازی شد که بیش‌ترین جدایه‌ها به ترتیب از خاک‌های جنگل، مزرعه، مرتع و باغ به دست آمد. با استفاده از ابزار جستجوی BLAST، توالی‌های آی.تی.اس. دی.ان.ای. ریبوزومی به دست آمده با سایر آرایه‌های معتبر مستخرج از بانک ژن مقایسه گردید.
در نهایت، گونه‌های Aspergillus nomius*، Fusarium oxysporum، F. equiseti،Fusarium sp.  ، Penicillium solitum،P. sizovae*
Penicillium sp.، Alternaria chlamydosporigena،Meyerozyma guilliermondii ،Paramyrothecium roridum ،
Chaetomium elatum،Beauveria bassiana ، B. pseudobassiana* وB. tenella*  شناسایی شدند. گونه‌های ستاره‌دار نخستین بار برای فلور قارچی ایران گزارش می‌شوند.
 
 

Keywords [Persian]

  • تاکسونومی
  • توالی ناحیه نسخه‌برداری شده داخلی دی.ان.ای. ریبوزومی
  • قارچ بیمارگر حشرات
  • کنترل بیولوژیک

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Rostaniha
Volume 21, Issue 1 - Serial Number 59
September 2020
Pages 49-64

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History

  • Receive Date: 17 March 2020
  • Revise Date: 13 May 2020
  • Accept Date: 16 June 2020

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