New records of fungi from rhizosphere of oak trees for Iran fungal flora

Document Type : Systematics and Biodiversity of Fungi

Authors

1 MSc Graduate in Plant Pathology, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Assistant Prof. of Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran

3 Prof. of Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran

4 Instructor of Microbial Biotechnology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education & Extension Organization (AREEO), Karaj, Iran

5 Prof. of Mycology and Plant Pathology, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj 31587-77871, Iran

Abstract

The objective of the present study was to isolate and characterize of fungal species from Persian oak trees rhizosphere in Zagros forests, Ilam province (Iran). For this purpose, sampling was performed from different regions of the above-mentioned forests during 2014–15. The serial dilutions of soil suspensions were cultured on Water agar (WA 2%), Potato dextrose agar (PDA), and Malt extract agar (MEA) media. Fungi were purified by transferring single hyphal tips on PDA medium.  At the first, the fungal isolates were identified using their morphological features. To confirm the morphological identification and taxonomic position of the isolates, molecular studied and phylogenetic analyses were then carried out using amplification and sequencing of ITS region of ribosomal DNA. Finally, the species Mortierella alpina, Penicillago nodositata,and Trichocladium jilongensis were identified based on morphological characters and molecular studies. These species were described and reported as new records for the mycobiota of Iran.  

 

Keywords


Article Title [Persian]

معرفی گونه‌های جدید قارچی از ریزوسفر درختان بلوط برای فلور قارچی ایران

Authors [Persian]

  • امین علیدادی 1
  • مژگان کوثری 2
  • غلامرضا صالحی جوزانی 3
  • ابراهیم کریمی 4
  • محمد جوان نیکخواه 5
  • سعدی کرمی 1
1 دانش‌آموخته کارشناسی ارشد بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران
2 استادیار بخش بیوتکنولوژی میکروبی، پژوهشگاه بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، ترویج و آموزش کشاورزی، کرج، ایران
3 استاد بخش بیوتکنولوژی میکروبی، پژوهشگاه بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، ترویج و آموزش کشاورزی، کرج، ایران
4 مربی بخش بیوتکنولوژی میکروبی، پژوهشگاه بیوتکنولوژی کشاورزی ایران، سازمان تحقیقات، ترویج و آموزش کشاورزی، کرج، ایران
5 استاد گروه گیاه‌پزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران
Abstract [Persian]

تحقیق حاضر با هدف جداسازی و شناسایی گونه‌های قارچی موجود در ریزوسفر درختان بلوط در جنگل‌های زاگرس استان ایلام انجام شد. برای این منظور، نمونه‌برداری از خاک ریزوسفر درختان بلوط در مناطق مختلف جنگل‌های زاگرس در استان ایلام طی سال‌های 94-1393 صورت پذیرفت. رقت‌های مختلفی از سوسپانسیون خاک تهیه و روی محیط‎کشت‌های عصاره مالت-آگار (MEA)، سیب‌زمینی-دکستروز-آگار (PDA) و عصاره آب-آگار (WA 2%) پخش گردید. خالص‌سازی جدایه‌ها با استفاده از روش نوک ریسه روی محیط‌کشت غذایی PDA انجام شد. ابتدا جدایه‌ها با استفاده از ویژگی‌های ریخت‌شناختی شناسایی شدند. سپس، به منظور تایید شناسایی ریخت‌شناختی و تعیین جایگاه تاکسونومیکی جدایه‌ها، شناسایی مولکولی و آنالیزهای فیلوژنتیکی با استفاده از ترادف‌های نوکلئوتیدی ناحیه ITS-rDNA صورت پذیرفت. در نهایت، براساس مطالعات ریخت‌شناختی و مولکولی، سه گونه شامل Mortierella alpina،Penicillago nodositataو Trichocladium jilongensis شناسایی شدند که در تحقیق حاضر به عنوان آرایه‌های جدید برای فلور قارچی ایران توصیف می‌شوند.
 

Keywords [Persian]

  • تاکسونومی
  • ریخت‎شناسی
  • Mortierella alpina
  • Penicillago nodositata
  • Trichocladium jilongensis
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