A preliminary report of aquatic hyphomycetes isolated from Anzali lagoon (Gilan province, North of Iran)

Document Type: Research Paper

Authors

1 Graduated MSc Student, Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Associate Prof., Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

3 PhD Student, Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

4 Prof., Escuela de Biologia, Universidad de Costa Rica, 11501 San Jose, Costa Rica

5 Prof., Department of Experimental Limnology, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Alte Fischerhuette 2, D-16775 Stechlin and Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany

6 Research Assistant Prof., Department of Botany, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

Abstract

Aquatic hyphomycetes equipped with a rich array of extracellular enzymes are involved in bioremediation of anthropogenic pollutants, plastics, aromatic contaminants and petroleum hydrocarbons. The current study contributes to the knowledge of Iranian aquatic hyphomycetes. Sampling was conducted in Anzali lagoon, Gilan province, Iran during 2017. Among 55 fungal isolates obtained during this study, eight species including Arthrobotrys oligosporus, Ceratorhiza hydrophila, Fusarium cf. ensiforme, F. incarnatum species complex, Myrmecridium schulzeri, Paecilomyces variotii, Sarocladium subulatum, and Volutella citrinella were identified based on their morphological characteristics. Furthermore, three species assigned to the genera Arthrobotrys, Fusarium and Sarocladium remained unidentified. Molecular studies using mainly ITS, LSU and SSU rDNA and in some cases β-tubulin (tub2) and translation elongation factor 1-a (tef1) determined the phylogenetic position of the isolates among closely related species. The occurrence of members of Fusarium, Sarocladium, and Ceratorhiza, known as rice disease agents, in freshwater ecosystems is interesting. This is the first report of aquatic hyphomycetes communities in Anzali lagoon, Iran. In this study, Sarocladium subulatum, Myrmecridium schulzeri, and Volutella citrinella are reported for the first time for Iran.
 
 

Keywords


Article Title [Persian]

گزارش مقدماتی از هیفومیست‌های آبزی تالاب انزلی (استان گیلان)

Authors [Persian]

  • فروغ رضاخانی 1
  • سید اکبر خداپرست 2
  • حسین مسیگل 3
  • کیلور روخاس-خیمنز 4
  • هانس-پیتر گروسارت 5
  • مونس بخشی 6
1 دانش‌آموخته کارشناسی ارشد، گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
2 دانشیار گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
3 دانشجوی دوره دکتری، گروه گیاه‌پزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
4 استاد بخش زیست‌شناسی، دانشگاه کاستاریکا، کاستاریکا
5 استاد بخش آب شیرین‌شناسی تجربی، مؤسسه اکولوژی آب شیرین و شیلات لیبنیتز (آی.جی.بی.)، آلمان
6 استادیار پژوهش بخش تحقیقات رستنی‌ها، مؤسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران
Abstract [Persian]

 
هیفومیست‌های آبزی تولید‌کننده تعدادی زیادی آنزیم‌های خارج سلولی هستند که در تجزیه زیستی آلودگی‌های ناشی از فعالیت‌های انسانی، پلاستیک، ترکیبات آروماتیک و هیدروکربن‌های نفتی نقش دارند. مطالعه حاضر با هدف افزایش دانش ما از هیفومیست‌های آبزی در ایران صورت گرفت. نمونه‌برداری طی سال 1396 از تالاب انزلی در استان گیلان انجام گرفت. از میان 55 جدایه قارچی که در این مطالعه به دست آمد، تعداد هشت گونه شامل Arthrobotrys oligosporus، Ceratorhiza hydrophila، Fusarium cf. ensiforme، F. incarnatum species complex، Myrmecridium schulzeri،Paecilomyces variotii، Sarocladium subulatum و Volutella citrinella برمبنای خصوصیات ریخت‌شناختی شناسایی شدند. علاوه براین، سه گونه متعلق به جنس‌های Arthrobotrys ، Fusarium  وSarocladium  ناشناخته باقی ماندند. شناسایی مولکولی اغلب گونه‌ها با استفاده از سه ناحیه ژنی شامل فاصله ترانویسی‌شده داخلی (ITS)، زیرواحد کوچک (SSU) و بزرگ (LSU) دی اِن اِی ریبوزومی (rDNA)، همچنین در مواردی از سازه امتداد ترجمه یک آلفا (tef1) و بتاتوبولین (tub2) موقعیت جدایه‌ها را در میان گونه‌های نزدیک نشان داد. حضور جنس‌هایی نظیر Fusarium، Sarocladium وCeratorhiza  در زیست‌بوم‌های آب شیرین که به عنوان عوامل بیماری‌زای برنج مطرح هستند، جالب توجه است. این نخستین گزارش از جمعیت‌های هیفومیستی در تالاب انزلی است. در این تحقیق، Sarocladium subulatum، Myrmecridium schulzeri و Volutella citrinella برای نخستین بار از ایران گزارش می‌شوند.
 
 

Keywords [Persian]

  • آلودگی‌های محیطی
  • تنوع زیستی
  • قارچ‌های آب‌های شیرین
  • قارچ‌های میتوسپوریک
  • قارچ‌های آنامورفیک
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