Solanum kieseritzkii and its comparison with S. dulcamara in Hyrcanian forests (North of Iran) based on morphological and molecular data

Document Type : Systematics and Biodiversity of Plants

Authors

1 PhD Student, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Research Prof., Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), P.O. Box 13185-116, Tehran, Iran

3 Research Associate Prof., Department of Botany, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran

4 Associate Prof., Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

 
Solanum kieseritzkii, an endemic species of Iran and the Caucasus, has been recently introduced in botanical sources as unresolved species and sometimes has been regarded a synonymous of S. dulcamara. In this research, along with field and herbarium studies, a morphological and molecular comparison is made between these two species; each of them is designated as a distinct species. Solanum kieseritzkii is a creeping rhizomatous  plant 10–30 cm high, has only 1–3 flowers per inflorescence, and is found only in the dark forests of Hyrcanian province, while S. dulcamara is a plant of open habitats including residential areas, climbing the tress up to 300 cm high, and has inflorescences with 6–40 flowers. The results of the present study were highly supported by comparison of internal transcribed spacer (ITS) sequences of different samples of both S. kieseritzkii and S. dulcamara and also comparing with other accessions from Genbank. Separation of two species is also confirmed by presented phylogenic tree.
 

Keywords

Main Subjects

Article Title [Persian]

مطالعه Solanum kieseritzkii و مقایسه آن با S. dulcamara در جنگل‌های هیرکانی (شمال ایران) براساس داده‌های مورفولوژیکی و مولکولی

Authors [Persian]

  • مجید اسکندری 1
  • مصطفی اسدی 2
  • سعید شیرزادیان 3
  • ایرج مهرگان 4
1 دانشجوی دکتری، گروه زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
2 استاد پژوهش، مؤسسه تحقیقات جنگل‌ها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، صندوق پستی 116-13185، تهران، ایران
3 دانشیار پژوهش، بخش تحقیقات رستنی‌ها، مؤسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران
4 دانشیار زیست‌شناسی و فیلوژنی مولکولی گیاهی، گروه زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
Abstract [Persian]

 
.Solanum kieseritzkii C.A.Mey یک گونه انحصاری ایران و قفقاز است که در منابع اخیر گیاه‌شناسی به عنوان گونه نامعین (unresolved) معرفی شده و اغلب با گونه S. dulcamara L. مترادف شده است. در این تحقیق، ضمن بررسی‌های میدانی و هرباریومی، مقایسه ریخت‌شناسی و مولکولی بین این دو گونه انجام شده و هر کدام به عنوان یک گونه مجزا اعلام می‌شوند. S. kieseritzkii  تنها در کف جنگل‌های تاریک هیرکانی و به صورت خزنده و ریزوم‌دار مشاهده می‌شود که ارتفاع گیاه اصلی بین 30-10 سانتی‌متر و دارای 3-1 گل و میوه می‌باشد، در حالی که گونه S. dulcamara با تفاوتی قابل ملاحظه‌ در مناطق باز و آفتابی و حتی در مناطق مسکونی، به ارتفاع تا 300 سانتی‌متر روییده و از گیاهان و درختان دیگر به عنوان تکیه‌گاه استفاده می‌کند. همچنین، تعداد گل و میوه این گیاه بین 40-6 عدد می‌باشد. در پژوهش حاضر، علاوه بر بررسی مورفولوژیکی، توالی ژنتیکی دو نمونه از گیاه S. kieseritzkii و دو نمونه از گیاه S. dulcamara با استفاده از ناحیه فاصله‌انداز رونویسی شونده درونی (ITS)، تهیه، ثبت و با توالی‌های مشابه در بانک ژن NCBI مقایسه و بررسی شدند. نتایج حاصل از داده‌های مولکولی نیز حاکی از تمایز فیلوژنتیکی دو گونه مورد نظر است.

 

 

Keywords [Persian]

  • انحصاری
  • پراکنش
  • تیره سیب زمینی
  • زیستگاه
  • سیستماتیک
  • فیلوژنی

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Rostaniha
Volume 20, Issue 2 - Serial Number 58
December 2019
Pages 110-122

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History

  • Receive Date: 07 July 2019
  • Revise Date: 24 August 2019
  • Accept Date: 11 September 2019

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