New records and hosts of the Curvularia species in Iran

Document Type : Systematics and Biodiversity of Fungi


1 MSc Student of Plant Pathology, Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Iran

2 Associate Prof. of Plant Pathology, Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Iran; Biotechnology and Bioscience Research Center, Shahid Chamran University of Ahvaz, Iran

3 Prof. of Plant Pathology, Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Iran


In present study, eight isolates of the genus Curvularia, recovered from plants of the cowpea, maize and bottlebrush in Khuzestan province, were identified based on molecular phylogeny in combination with morphology. The mycelial biomass of the isolates, produced in potato-dextrose-Broth, were collected by passing through filter papers and freeze-dried. DNA was extracted using a phenol- and chloroform-based method. The partial regions of nrDNA and gpd were amplified using the appropriate primers and then sequenced. The consensus ITS and gpd sequences of the isolates under survey were compared to reference sequences of the type strains in GenBank by BLASTn algorithm and two-loci phylogenetic analysis. Accordingly, the isolates were identified at species level as follow: Curvularia americana, C. muehlenbeckiae,and C.verruculosa. To our knowledge, this is the first record of both the C. americana and C. muehlenbeckiae in Iran. Moreover, this study reports new hosts for all three species with further morphological and molecular characterization of C.verruculosa species as well.


Main Subjects

Article Title [فارسی]

گزارش‌ها و میزبان‏‌‌‌‌‌های جدید گونه‏‌‌‌‌‌‏‌‌‌‌‌های Curvularia در ایران

Authors [فارسی]

  • سحر جانبزرگی 1
  • مهدی مهرابی کوشکی 2
  • رضا فرخی نژاد 3
1 دانشجوی کارشناسی‏‌‌‌‌‌ارشد بیماری‌شناسی گیاهی، گروه گیاه‏‌‌‌‌‌پزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، ایران
2 دانشیار بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، ایران؛ گروه بیوتکنولوژی، مرکز تحقیقات بیوتکنولوژی و علوم زیستی، دانشگاه شهید چمران اهواز، ایران
3 استاد بیماری‏‌‌‌‌‌شناسی گیاهی، گروه گیاه‏‌‌‌‌‌پزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، ایران
Abstract [فارسی]

در مطالعه حاضر، هشت جدایه‏‌‌‌‌‌ از جنس Curvularia، جداسازی شده از گیاهان لوبیا چشم‌بلبلی، ذرت و شیشه‌شور در استان خوزستان، براساس روش‏‌‌‌‌‌ مبتنی بر فیلوژنی مولکولی و ریخت‏‌‌‌‌‌شناسی شناسایی شدند. زیست‏‌‌‌‌‌توده‌ میسلیومی جدایه‏‌‌‌‌‌ها، تولید شده در محیط مایع سیب زمینی-دکستروز، به وسیله‏‌‌‌‌‌ کاغذ صافی جمع‏‌‌‌‌‌آوری و خشک-‏‌‌‌‌‌انجمادی شد. توده‏‌‌‌‌‌ DNA با استفاده از یک روش مبتنی بر فنل و کلروفرم استخراج شد. بخش‏‌‌‌‌‌هایی از نواحی rDNA هسته‏‌‌‌‌‌ای و gpd با استفاده از آغازگر‏‌‌‌‌‌های مناسب تکثیر و سپس توالی‏‌‌‌‌‌یابی شدند. توالی‏‌‌‌‌‌های ITS و gpd مربوط به جدایه‏‌‌‌‌‌های تحت تحقیق با توالی‏‌‌‌‌‌های معتبر مربوط به سویه‏‌‌‌‌‌های تیپ در بانک ژن با استفاده از الگوریتم BLASTn و تجزیه و تحلیل فیلوژنتیکی (مبتنی بر دو ناحیه‏‌‌‌‌‌ ژنومی) مورد مقایسه قرار گرفتند. بر این اساس، جدایه‏‌‌‌‌‌ها در سطح گونه شناسایی و گونه‏‌‌‌‌‌هایC. americana، C. muehlenbeckiae و C.verruculosa را شامل شدند. لذا، این نخستین گزارش دو گونه‏‌‌‌‌‌ C. americana و C. muehlenbeckiae در ایران می‏‌‌‌‌‌باشد. به علاوه، این مطالعه برای هر سه گونه‌‌‌‌‌ مورد بررسی میزبان جدیدی گزارش می‏‌‌‌‌‌کند و مشخصات ریخت‏‌‌‌شناسی و مولکولی بیشتری برای گونه‌ C.verruculosa ارایه می‏‌‌‌‌‌دهد.

Keywords [فارسی]

  • ریخت شناسی
  • فیلوژنی مولکولی
  • ذرت
  • شیشه شور
  • لوبیا چشم بلبلی
Ahmadpour, A., Heidarian, Z., Karami, S., Pordel, A., Jabbarifar, S.M. & Javan-Nikkhah, M. 2013. New species of Bipolaris and Curvularia on poaceous plants in Iran. Rostaniha 14: 216–228.
Ahmadpour, S.A., Mehrabi-Koushki, M. & Farokhinejad, R. 2017. Neodidymelliopsis farokhinejadii, a new fungal species from dead branches of trees in Iran. Sydowia 69: 171–182.
Almaguer, M., Rojas, T.I., Dobal, V., Batista, A. & Aira. M.J. 2013. Effect of temperature on growthand germination of conidia in Curvularia and Bipolaris species isolated from the air. Aerobiologia29: 13–20.
Babaahmadi, G., Mehrabi-Koushki, M. & Hayati,J. 2018. Allophoma hayatii sp. nov., an undescribed pathogenic fungus causing dieback of Lantana camarain Iran. Mycological Progress 17(3): 365–379.
Beneke, E.S. & Rogers, A.L. 1996. Medical mycology and human mycoses. Star Publishing Company, Belmont, 239 pp.
Berbee, M., Pirseyedi, M. & Hubbard, S. 1999. Cochliobolus phylogenetics and the origin of known, highly virulent pathogens, inferred
from ITS and glyceraldehyde-3-phosphate dehydrogenase gene sequences. Mycologia 91: 964–977.
Boedijn, K.B. 1933. Ueber einige phragmosporen Dematiazeen. Bulletin du Jardin botanique de Buitenzorg 13: 120–134.
Dehdari, F., Mehrabi-Koushki, M. & Hayati, J. 2018. Curvularia shahidchamranensis sp. nov., a crude oil-tolerant fungus. Current Research in Environmental and Applied Mycology 8: 572–584.
Ershad, D. 2009. Fungi of Iran, 3rd edn. Iranian Research Institute ofPlant Protection, Tehran.
Gautam, A.K., Kant, M. & Thakur, Y. 2013. Isolation of endophytic fungi from Cannabis sativa and study their antifungal potential. Archive of Phytopathology and Plant Protection46: 627–635.
Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
Hargreaves, M., Parappukkaran, S., Morawska, L., Hitchins, J., He, C. & Gilbert, D. 2003. A pilot investigation into associations between indoor airborne fungal and non-biological particle concentrations in residential houses in Brisbane, Australia. Science of Total Environment 312: 89–101.
Heidari, K., Farokhinejad, R. & Mehrabi-Koushki, M. 2018a. Occurrence of purslane leaf spot caused by Dichotomophthora lutea in Iran. Australasian Plant Disease Notes 13(1): 1–4.
Heidari, K., Mehrabi-Koushki M. & Farokhinejad R. 2018b. Curvularia mosaddeghii sp. nov., a novel species from the family Pleosporaceae. Mycosphere 9: 635–646.
Hibbett, D.S., Binder, M., Bischoff, J.F., Blackwell, M., Cannon, P.F., Eriksson, O.E., Huhndorf, S., James, T., Kirk, P.M., Stalpers, J.A., Crous, P.W., Gams, W. et al. 2007. A higher-level phylogenetic classification of the Fungi. Mycological Research 111: 509–547.
Jena, S.K. & Tayung, K. 2013. Endophytic fungal communities associated with two ethno-medicinal plants of Similipal Biosphere Reserve, India and their antimicrobial prospective. Journal of Applied Pharmaceutical Science 3: 7.
Liang, Y., Ran, S.F., Bhat, J., Hyde, K.D., Wang, Y. & Zhao, D.J. 2018. Curvularia microspora sp. nov. associated with leaf diseases of Hippeastrum striatum in China. MycoKeys 29: 49–61.
Madrid, H., da Cunha, K.C., Gene, J., Dijksterhuis, J., Cano, J., Sutton, D.A., Guarro, J. & Crous, P.W. 2014. Novel Curvularia species from clinical specimens. Persoonia 33: 48–60.
Manamgoda, D.S., Cai, L., Bahkali, A.H., Chukeatirote, E. & Hyde, K.D. 2011. Cochliobolus: an overview and current status of species. Fungal Diversity 51: 3–42.
Manamgoda, D.S., Cai, L., McKenzie, E.H., Crous, P.W., Madrid, H., Chukeatirote, E., Shivas, R.G., Tan, Y.P. & Hyde, K.D. 2012. A phylogenetic and taxonomic re-evaluation
of the Bipolaris-Cochliobolus-Curvularia complex. Fungal Diversity 56: 131–44.
Manamgoda, D.S., Cai, L., McKenzie, E.H.C., Chukeatirote, E. & Hyde, K.D. 2012. Two new Curvularia species from northern Thailand. Sydowia 64: 255–266.
Manamgoda, D.S., Rossman, A.Y., Castlebury, L.A., Chukeatirote, E. & Hyde, K.D. 2015. taxonomic and phylogenetic re-appraisal of the genus Curvularia (Pleosporaceae): human and plant pathogens. Phytotaxa 212: 175–198.
Manamgoda, D.S., Rossman, A.Y., Castlebury, L.A., Crous, P.W., Madrid, H., Chukeatirote, E. & Hyde, K.D. 2014. The genus Bipolaris. Studies in Mycology 79: 221–288.
Marin-Felix, Y., Groenewald, J.Z., Cai, L., Chen, Q., Marincowitz, S., Barnes, I., Bensch, K., Braun, U., Camporesi, E., Damm, U., de Beer, Z.W., Dissanayake, A., Edwards, J., Giraldo, A., Hernández-Restrepo, M., Hyde, K.D., Jayawardena, R.S., Lombard, L., Luangsa-ard, J., McTaggart, A.R., Rossman, A.Y., Sandoval-Denis, M., Shen, M., Shivas, R.G., Tan, Y.P., van der Linde, E.J., Wingfield, M.G.,  Wood, A.R., Zhang, J.Q., Zhang Y. & Crous, P.W. 2017a. Genera of phytopathogenic fungi: GOPHY 1. Studies in Mycology 86: 99–216.
Marin-Felix, Y., Senwanna, C., Cheewangkoon, R. & Crous, P.W. 2017b. New species and records of Bipolaris and Curvularia from Thailand. Mycosphere 8: 1556–1574.
Mehrabi-Koushki, M., Pooladi, P., Eisvand, P. & Babaahmadi, G. 2018. Curvularia ahvazensis and C. rouhanii spp. nov. from Iran. Mycosphere 9: 1173–1186.
O’Donnell, K. 1993. Fusarium and ITS near relatives, pp. 225–233. In: Reynolds, D.R. & Taylor, J.W. (eds). The fungal holomorph: mitotic, meiotic and pleomorphic speciation in fungal systematics. CABI Publishing, Wallingford.
Raeder, U. & Broda, P. 1985. Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology 1: 17–20.
Scott, E.M. & Carter, R.T. 2014. Canine keratomycosis in 11 dogs: A case series (2000–2011). Journal of the American Animal Hospital Association 50: 112–118.
Sharma, R., Prakash, O., Sonawane, M.S., Nimonkar, Y., Golellu, P.B. & Sharma, R. 2016. Diversity and distribution of phenol oxidase producing fungi from soda lake and description of Curvularia lonarensis sp. nov. Frontiers in Microbiology 7: 1847.
Tadych, M., Bergen, M., Johnson, J.C., Polashock, J. & Vorsa, N. 2012. Endophytic and pathogenic fungi of developing cranberry ovaries from flower to mature fruit: diversity and succession. Fungal Diversity 54: 101–116.
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30: 2725–2729.
Tan, Y.P., Madrid, H., Crous, P.W. & Shivas, R.G. 2014. Johnalcornia gen. et. comb. nov., and nine new combinations in Curvularia based on molecular phylogenetic analysis. Australasian Plant Pathology 43: 589–603.
Tan, Y.P., Crous, P.W. & Shivas R.G. 2018. Cryptic species of Curvularia in the culture collection of the Queensland Plant Pathology Herbarium. MycoKeys 35: 1–25.
Tomaso-Peterson, M., Jo, Y.K., Vines, P.L. & Hoffmann, F.G. 2016. Curvularia malina sp. nov. incites a new disease of warm-season turf grasses in the southeastern United States. Mycologia 108: 915–924.
Verma, P., Singh, S. & Singh, R. 2013. Seven species of Curvularia isolated from three lakes of Bhopal. Advances in Life Science and Technology 8: 13–15.
White, T.J., Bruns, T., Lee, S. & Taylor, J.L. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, pp. 315–322. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (eds). PCR protocols: a guide to methods and applications. Academic Press.