Morphological phylogenetic analysis of the genera Fragaria and Duchesnea in Iran

Document Type : Research Paper

Authors

1 Associate Prof., Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran

2 MSc Student, Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran

3 Prof., School of Biology, University College of Science, University of Tehran, Tehran, Iran

Abstract

In this research phylogenetic relationships of the two genera Fragaria and Duchesnea, including four species ( Fragaria viridis, F. vesca, Duchesnea indica and D. chrysantha) and 2 of their closely related species (Potentilla reptans and P. micrantha) plus 2 Fillipendulla species ( representing outgroups) were carried out using morphological traits. Primarily, morphological evidences of 30 taxa were carefully examined. Then, phylogenetic analysis was conducted in two steps, 1) using 38 quantitative and qualitative characters 2) using 10 selected evidences. For this, all studied morphological characters were coded and analyzed by Maximum parsimony method (MP). Data analysis was carried out through the heuristic search embedded in the PAUP software. The result emphasizes the separation of the two subtribe Potentillinae and Fragariinae. It also shows monophyly of Fragaria and Duchesnea. Furthermore, based on the current findings, Potentilla is a closest relative of Duchesnea. Among the studied morphological characters14 (9 from the first and 5 from second analysis) isolates Fragaria from Duchesnea; and 6 (4 from the first analysis and 2 from the second analysis) separates Duchesnea from Potentilla. However, intra-species relationships between the Fragaria species) except for 4 taxa) remained unresolved.

Keywords

Main Subjects

Article Title [Persian]

مطالعه فیلوژنتیک ریخت شناختی جنس های Fragaria و Duchesnea از تیره گل سرخیان در ایران

Authors [Persian]

  • مرضیه بیگم فقیر 1
  • شیما پورابراهیم 2
  • فریده عطار 3
1 دانشیارگروه زیست شناسی، دانشکده علوم، دانشگاه گیلان،رشت، ایران
2 دانشجوی کارشناسی ارشد، گروه زیست شناسی، دانشکده علوم، دانشگاه گیلان، رشت، ایران
3 ستاد دانشکده زیست شناسی، پردیس علوم، دانشگاه تهران، تهران، ایران
Abstract [Persian]

در این تحقیق روابط فیلوژنتیکی دو جنس Fragaria و Duchesnea، چهار گونه) Fragaria viridis، F. vesca، Duchesnea indica و D. chrysantha ) و 2 گونه خویشاوند نزدیک آنها (P. micrantha و Potentilla reptans ) و 2 گونه Fillipendulla ( به عنوان برون گروه)، با استفاده از صفات مورفولوژیکی مورد بررسی قرار گرفت. ابتدا صفات ریخت شناسی 30 تاکسون به دقت مورد ارزیابی قرار گرفت.سپس تحلیل فیلوژنتیک در دو مرحله 1) با استفاده از 38 صفت کمی و کیفی و سپس 10 صفت انتخابی انجام شد. برای این کار صفات ریخت شناسی کدگذاری شدند و داده ها را به روش بیشینه پارسییمون (MP) مورد تجزیه و تحلیل قرار گرفتند.تحلیل داده ها از طریق جستجوی ابتکاری تعبیه شده در نرم افزار PAUP انجام شد. نتیجه این تحقیق بر جدایی دو زیرطایفه Potentillinae و Fragariinae تأکید دارد. مچنین بیانگر تک نیا یی Fragaria و Duchesnea می باشد. بعلاوه بر اساس یافته های این بررسیPotentilla نزدیکترین خویشاوند Duchesnea می باشد. از میان صفات مورد مطالعه بررسی در مجموع 14 صفت ( شامل 9 صفت از تحلیل اول و 5 صفت از تجزیه دوم) موجب جداسازی Fragaria از Duchesnea و 6 صفت ( 4 صفت از اولین تحلیل و2 صفت از تجزیه دوم) سبب تفکیک Duchesnea از Potentilla شدند. با این حال، روابط درون گونه ایی دو گونه ی Fragaria ( به غیر از 4 تاکسون) حل نشده باقی ماند.

Keywords [Persian]

  • تحلیل کلادیستیک
  • توت فرنگی
  • توت فرنگی کاذب
  • گونه های خویشاوند
  • بیشینه پارسیمونی

References

Adanson, M. 1763. Familles des plantes. Paris. Chez Vincent. Imprimeur-Librarie de Mgr le. Comte de Provence, rue S. Servin.
Bringhurst, R.S. & Senanayake, Y.D.A. 1966. The evolutionary significance of natural Fragaria chiloensis× F. vesca hybrids resulting from unreduced gametes. American Journal of Botany 53(10): 1000–1006.
Darrow, G.M. 1966. The Strawberry: History, Breeding and Physiology. Holt, Rinehart and Winston, New York.
DiMeglio, L.M., Staudt, G., Yu, H. & Davis, T.M. 2014. A phylogenetic analysis of the genus Fragaria (strawberry) using intron-containing sequence from the ADH-1 gene. PLoS One 9(7): e102237.
Debes, M.A., Arias, M.E., Grellet-Bournonville, C.F., Wulff, A.F., Martínez-Zamora, M.G., Castagnaro, A.P. & Díaz-Ricci, J.C. 2011. White-fruited Duchesnea indica (Rosaceae) is impaired in ANS gene expression. American Journal of Botany 98(12): 2077–2083.
Dobeš, C. & Paule, J. 2010. A comprehensive chloroplast DNA-based phylogeny of the genus Potentilla (Rosaceae): Implications for its geographic origin, phylogeography and generic circumscription. Molecular Phylogenetic and Evolution 56: 156–175.
Domac, R. 1994. Flora Hrvatske. Priručnik za određivanje bilja. Školska knjiga, Zagreb.Eriksson, T., Donoghue, M.J. & Hibbs, M.S. 1998. Phylogenetic analysis of Potentilla using DNA sequences of nuclear ribosomal internal transcribed spacer (ITS), and its implications for the classification of Rosoideae (Rosaceae). Plant Systematic and Evolution 11: 155–179.
Eriksson, T., Hibbs, M.S., Yoder, A.D., Delwiche, C.F. & Donoghue, M.J. 2003. The phylogeny of Rosoideae (Rosaceae) based on sequences of the internal transcribed spacers (ITS) of nuclear ribosomal DNA and the trnL/F region of chloroplast DNA. Intentional Journal of Plant Science 164: 197–211.
Faghir, M.B., Attar, F., Farazmand, A. & Kazempur Osaloo, Sh. 2014. Phylogeny of the genus Potentilla (Rosaceae) in Iran based on nrDNA ITS and cpDNAtrnL-F sequences with a focus on leaf and style characters’ evolution. Turkish Journal of Botany 38: 417–429.
Farris, J.S. 1969. A successive approximation approach to character weighting. Systematic Zoology 18: 374–385.
Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783–791.
Hancock, J.F., Lavín, A. & Retamales, J.B. 1999. Our southern strawberry heritage: Fragaria chiloensis of Chile. Horticultural Science 34(5): 814–816.
Harrison, R.E., Luby, J.J. & Furnier, G.R. 1997. Chloroplast DNA restriction fragment variation among strawberry (Fragaria spp.) Taxa. Journal of the American Society for Horticultural Science 122(1): 63–68.
Hebda, R.J. & Chinnappa, C.C. 1994. Studies on pollen morphology of Rosaceae. Acta Botanica Gallica 141(2): 183–193.
Heo, K., Kim, Y., Maki, M. & Park, J. 2019. The complete chloroplast genome of mock strawberry, Duchesnea indica (Andrews) Th. Wolf (Rosoideae). Journal of Mitochondrial DNA Part B. Resources 4(1): 560–562. https://doi.org/10.1080/23802359.2018.1553527.
Hutchinson, J. 1964. The genera of flowering plants, Vol. 1, Dicotyledons. Clarendon Press, Oxford.
Hummer, K.E., Nathewet, P. & Yanagi, T. 2009. Decaploidy in Fragaria iturupensis (Rosaceae). American Journal of Botany 96(3): 713–716.
Hummer, K.E. & Janick, J. 2009. Rosaceae: taxonomy, economic importance, genomics. Pp. 1–17. In: Genetics and genomics of Rosaceae. Springer, New York.
Hummer, K.E., Bassil, N. & Njuguna, W. 2011. Fragaria In Wild Crop Relatives: Genomic and Breeding Resources. Springer, Berlin, Heidelberg. Pp. 17–44.
Juzepczuk, S.W. 1941. Alchemilla. In: Komarov, V.L. (ed.). Flora of USSR, Vol. 10, Izd Akad Nauk SSSR, Moskva Leningrad. Pp. 289–410.
Khatamsaz, M. 1993. Flora of Iran. Rosaceae. Research Institute of Forests and Rangeland Press. Tehran 6: 88–140.
Linnaeus, C. 1753. Fragaria (Rosaceae). P. 492. In: Species Plantarum, Vol. 2. Laurentius Salvius, Stockholm.
Lei, J., Li, Y., Du, G., Dai, H. & Deng, M. 2005. A natural pentaploid strawberry genotype from the Changbai Mountains in northeast China. Horticultural Science 40: 1194–1195.
Lewis, P.O. 2001. A likelihood approach to estimating phylogeny from discrete morphological character data. Systematic Biology50: 913–925.
Metcalfe, C.R. & Chalk, L. 1957. Anatomy of dicotyledons II. Claredon Press, Oxford.
Maddison, W.P., Donoghue, M.J. & Maddison, D.R. 1984. Outgroup analysis and parsimony. Systematic Zoology 33: 83–103.
Morgan, D.R., Soltis, D.E. & Robertson, K.R. 1994. Systematic and evolutionary implication of rbCL sequence variation in Rosaceae. American Journal of Botany 12: 890–903.
Naruhashi, N. & Sugimoto, M. 1996. The floral biology of Duchesnea (Rosaceae). Plant Species Biology 11(3): 173–184.
Naruhashi, N., Iwatsubo, Y. & Peng, C.I. 1999. Cytology, flower morphology and distribution of Fragaria hayatai Makino (Rosaceae). Journal of Phytogeography and Taxonomy 47: 139–143.
Nylander, J.A., Ronquist, J.P., Huelsenbeck, F. & Nieves-Aldrey, J.L. 2004. Bayesian phylogenetic analysis of combined data. Systematic Biology 53: 47–67.
Oda, Y. & Nishimura, K. 2009. Ichigo-Japanese name of strawberry- its origin and historical change of phonogram for strawberry in Japan. Acta Horticulturae 842: 611–618.
Olbricht, K., Ludwig, A., Ulrich, D., Spangenberg, R., Guenther, M. & Neinhuis, C. 2012. Leaf morphology and anatomy in the genus Fragaria: implications for resistances. VII International Strawberry Symposium 1049. Pp. 269–273.
Pardo, E.M., Grellet, C.F., Salazar, S.M., Castagnaro, A.P., Diaz Ricci, J.C. & Arias, M.E. 2012. Histopathology of the resistance to Colletotrichum gloeosporioides of wild strawberries and species related to commercial strawberry. Australian Journal of Crop Science 6(7): 1147–1153.
Potter, D., Luby, J.J. & Harrison, R.E. 2000. Phylogenetic relationships among species of Fragaria (Rosaceae) inferred from non-coding nuclear and chloroplast DNA sequences. Systematic Botany 25(2): 337–348.
Potter, D., Eriksson, T., Evans, R.C., Oh, S., Smedmark, J.E.E., Morgan, D.R. & Campbell, C.S. 2007. Phylogeny and classification of Rosaceae. Plant Systematics and Evolution 266: 5–43.
Pourebrahim, S., Faghir, M.B. & Attar, F. 2018. Report of new species of Duchesnea indica from Rosaceae for Guilan province. The International Conference on Agricultural Science. Medicinal Plants and Traditional Medicine. Mashhad, Iran.
Purgar, D.D., Duralija, B., Vokurka, A., Todorić, M. & Židovec, V. 2017. The Genus Fragaria in Croatia. Die Gattung Fragaria in Kroatien. Erwerbs-Obstbau 59(3): 227–233.
Retamales, J.B., Caligari, P.D.S., Carrasco, B. & Saud, G. 2005. Status of the Chilean native strawberry and the research needs to convert the species into a commercial crop. Horticultural Science 40: 1633–1644.
Rousseau-Gueutin, M., Gaston, A., Aïnouche, A., Aïnouche, M.L., Olbricht, K., Staudt, G. & Denoyes-Rothan, B. 2009. Tracking the evolutionary history of polyploidy in Fragaria L. (strawberry): new insights from phylogenetic analyses of low-copy nuclear genes. Molecular Phylogenetic and Evolution 51(3): 515–530.
Schönbeck-Temesy, H. 1969. Duchesnea (Rosaceae). Pp. 77–78. In: Flora Iranica, Vol. 66 (Rechinger, K.H., ed.). Akademische Druck u. Verlagsanstalt, Graz.
Soják, J. 2004. Potentilla L. (Rosaceae) and related genera in the former USSR. Notes on Potentilla XVI. Botanische Jahrbücher für Systematik 125: 253–340.
Soják, J. 2008. Notes on Potentilla 21. A new division of the tribe Potentilleae (Rosaceae) and notes on generic delimitations. Botanische Jahrbücher für Systematik 127: 349–358.
Soják, J. 2010. Argentina hill, a genus distinct from Potentilla (Rosaceae). Thaiszia Journal of Botany Košice 20: 91–97.
Soják, J. 2012. Potentilla L. (Rosaceae) and related genera in Asia (excluding the former USSR), Africa and New Guinea-Notes on Potentilla XXVIII. Plant Diversity and Evolution 130: 7–157.
Staudt, G. 1989. The species of Fragaria, their taxonomy and geographical distribution. Acta Horticulturae 265: 23– 33.
Staudt, G. 2003. Notes on Asiatic Fragaria species: III. Fragaria orientalis Losinsk and Fragaria mandshurica sp. nov. Botanische Jahrbücher für Systematik 124(4): 397–419.
Staudt, G. 2006. Himalayan species of Fragaria (Rosaceae). Botanische Jahrbücher für Systematik 126(4): 483–508.
Staudt, G. 2008. Strawberry Biogeography, Genetics and Systematics. VI International Strawberry Symposium, International Society for Horticultural Science. Huelva, Spain.
Staudt, G.S., Schneider, S., Scheewe, P. Ulrich, D. & Olbricht, K. 2008. Fragaria iturupensis, a new source for strawberry improvement. VI International Strawberry Symposium 842: 479–482.
Töpel, M., Lundberg, M., Eriksson, T. & Eriksen, B. 2011. Molecular data and ploidal levels indicate several putative allopolyploidization events in the genus Potentilla (Rosaceae). PLoS Currents 3.

Wolf, F.T. 1908. Monographie der Gattung Potentilla. Bibliotheca Botanica 16: 1–713.

Xiang, Y., Huang, C., Hu, Y., Wen, J., Li, S., Yi, T., Chen, H., Xiang, J. & Ma, H. 2016. Evolution of Rosaceae fruit types based on nuclear phylogeny in the context of geological times and genome duplication. Molecular Biology and Evolution 34(2): 262–281.
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Zare, H., Ramezani, K.E. & Amini, T. 2007. A record of Duchesnea indica (Rosaceae) in Iran, its westernmost distributional limit in Asia. Iranian Journal of Botany 13(2): 93–94.
Zhang, S.D., Jin, J.J., Chen, S.Y., Chase, M.W., Soltis, D.E., Li, H.T., Yang, J.B., Li, D.Z. & Yi, T.S. 2017. Diversification of Rosaceae since the late cretaceous based on plastid phylogenomics. New Phytologist 1–13.
Rostaniha
Volume 19, Issue 2 - Serial Number 56
December 2018
Pages 154-164

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History

  • Receive Date: 06 October 2018
  • Revise Date: 31 October 2018
  • Accept Date: 19 December 2018

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