Molecular phylogeny and morphology of four Ramularia species from Iran along with a checklist of ramularia-like taxa

Document Type: Systematics and Biodiversity of Fungi


Department of Botany, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran


The genus Ramularia includes important plant pathogens with worldwide distribution, commonly associated with leaf spot diseases on a broad range of plant hosts. Although these fungi are common in Iran, most of the species found to date have been identified on the basis of morphological characteristics, and DNA data are available for limited number of them. During our investigation of fungi associated with leaf spot diseases in north and northwest of Iran, Ramularia isolates were recovered from leaves with leaf spots on different herbaceous and woody plants in the Asteraceae, Apiaceae, and Vitaceae families. Based on sequence data of five genomic loci (ITS, actA, tef1, rpb2 and gapdh), host, cultural and morphological data; four species including R. cynarae on Cirsium arvense,R. heraclei on Heracleum sp.,R. hydrangeae-macrophyllae on Vitis vinifera,and R. inaequalis on Taraxacum campylodes, were identified. Ramularia hydrangeae-macrophyllae represents a new record for the mycobiota of Iran as well as Asia, and V. vinifera is a new host for this species in the world. Moreover, C. arvense and T. campylodes are new hosts for R. cynarae and R. inaequalis in Iran,respectively. Additionally, a comprehensive literature-based checklist for 50 ramularia-like species known to occur on different plant species in Iran was provided. The complete annotated list covers 41 Ramularia species, two Cercosporella, two Neoovularia, two Neoramularia,one Microcyclosporella, one Neopseudocercosporella, and one Ramulariopsis.


Main Subjects

Article Title [Persian]

فیلوژنی مولکولی و ریخت‌شناسی چهار گونه رامولاریا از ایران به همراه فهرستی از آرایه‌های شبه‌رامولاریا

Author [Persian]

  • مونس بخشی
عضو هیئت علمی بخش تحقیقات رستنی‌ها، مؤسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایرا
Abstract [Persian]

جنس Ramularia بیمارگرهای گیاهی مهمی را شامل می‌شود که در سراسر جهان پراکنده‌اند و اغلب با علایم لکه‌برگی در دامنه گسترده‌ای از میزبان‌های گیاهی همراه هستند. با وجود پراکنش گسترده این قارچ‌ها در ایران، تاکنون بیشتر گونه‌ها براساس صفات ریخت‌شناختی شناسایی شده‌اند و توالی DNA برای تعداد بسیار اندکی از آن‌ها وجود دارد. طی مطالعه عوامل ایجاد لکه برگی روی گیاهان مختلف در نواحی شمال و شمال‌غرب کشور، جدایه‌هایی از جنس رامولاریا از برگ‌های دارای علایم لکه‌برگی میزبان‌های چوبی و علفی در تیره‌های Asteraceae، Apiaceae و Vitaceae به دست آمد. براساس ترکیب داده‌های توالی پنج ناحیه ژنی (ITS، actA، tef1، rpb2و gapdh)، اطلاعات میزبان و صفات ریخت‌شناختی، چهار گونه شامل: R. cynarae روی کنگر صحرایی (CirsiumarvenseR. heracleiروی گیاهHeracleum sp.، R. hydrangeae-macrophyllae روی انگور (Vitis vinifera) و R. inaequalisروی گیاه گل قاصد (Taraxacum campylodes) شناسایی شدند. این نخستین گزارش از وجود آرایه R. hydrangeae-macrophyllae برای میکوبیوتای ایران و همچنین قاره آسیا بوده و انگور به عنوان میزبان جدیدی برای این گونه در دنیا گزارش می‌شود. همچنین، کنگر صحرایی و گل قاصد به ترتیب به عنوان میزبان‌های جدیدی در ایران برای دو گونه R. cynarae و R. inaequalis هستند.
علاوه‌بر‌این، در این پژوهش، فهرست کاملی از 50 گونه رامولاریا و جنس‌های مشابه که روی میزبان‌های مختلف در ایران گزارش شده‌اند، ارایه گردیده است. این فهرست دربردارنده 41 گونه از جنس رامولاریا، دو گونه از جنس Cercosporella، دو گونه از جنسNeoovularia، دو گونهNeoramularia،یک گونه Microcyclosporella،یک گونه Neopseudocercosporella و یک گونه Ramulariopsis می‌باشد.

Keywords [Persian]

  • سیستماتیک
  • فیلوژنی
  • قارچ‌های عامل لکه‌برگی
  • میکوسفرلا
  • هیفومیست
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