A taxonomic study of cyanobacteria in wheat fields adjacent to industrial areas in Yazd province (Iran)

Document Type : Research Paper

Authors

1 MSc Student, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, G.C. Tehran, Iran

2 Prof., Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, G.C. Tehran, Iran

3 Assistant Prof., Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, G.C. Tehran, Iran

4 Assistant Prof., Faculty of Natural Resource, Yazd University, Yazd, Iran

Abstract

Culturing, isolation, purification, and identification of cyanobacteria collected from wheat field soil, in five stations around the industrial areas in Yazd province (Iran) were conducted in this study. Identification of taxa was based on morphology and molecular methods. Cluster analysis and principal component analyses performed using SPSS software and rate of resemblance among the taxa were investigated. The phylogenetic analyses carried out according to 16S rRNA gene sequences data, and the phylogenetic relationships were assessed by Maximum Parsimony, Maximum Likelihood and Bayesian Inference. The results of this study indicated presence of 32 species included in four orders of cyanobacteria. Among these orders, Oscillatoriales with three families, eight genera and 13 species showed the highest species diversity, whereas Chroococcales with two families, three genera and four species, exhibited the lowest diversity. Among isolated taxa, Oscillatoria ornata, O. engelmanniana, Jaaginema pallidum, Pseudanabaena minima,Stenomitos frigidus, Nostoc edaphicum, and Gloeothece tepidariorum, are reported as new records for algal flora in Iran. Distributions and descriptions of the new taxa were also presented.

Keywords

Article Title [Persian]

مطالعه تاکسونومیک سیانوباکتری‌های مزارع گندم مجاور مناطق صنعتی استان یزد

Authors [Persian]

  • مریم اهل سعادت 1
  • حسین ریاحی 2
  • زینب شریعتمداری 3
  • ﻣﺤﻤﺪ ﺣﺴﯿﻦ ﺣﮑﯿﻤﯽ ﻣﯿﺒﺪی 4
1 کارشناسی ارشد داﻧﺸﮑﺪه ﻋﻠﻮم و فناوری زﯾﺴﺘﯽ، دانشگاه شهید بهشتی، تهران
2 استاد داﻧﺸﮑﺪه ﻋﻠﻮم و فناوری زیستی، دانشگاه شهید بهشتی، تهران
3 استادیار داﻧﺸﮑﺪه ﻋﻠﻮم و فناوری زﯾﺴﺘﯽ، دانشگاه شهید بهشتی، تهران
4 اﺳﺘﺎدﯾﺎر داﻧﺸﮑﺪه ﻣﻨﺎﺑﻊ ﻃﺒﯿﻌﯽ، داﻧﺸﮕﺎه ﯾﺰد، ﯾﺰد
Abstract [Persian]

در این مطالعه، سیانوباکتری‌های موجود در خاک مزارع گندم در پنج ایستگاه در مجاورت مناطق صنعتی استان یزد پس از طی مراحل کشت و خالص‌سازی مورد شناسایی قرار گرفتند. شناسایی نمونه‌ها براساس ویژگی‌های مورفولوژیک و نیز به روش مولکولی صورت گرفت. همچنین تجزیه خوشه‌ای و تجزیه مولفه‌های اصلی با استفاده از نرم‌افزار SPSS صورت گرفت و قرابت میان آرایه‌ها براساس صفات ریختی بررسی گردید. همچنین آنالیزهای فیلوژنتیک با استفاده از مارکر مولکولی 16S rRNA انجام و روابط فیلوژنتیک آرایه‌ها براساس روش‌های Maximum Parsimony، Maximum Likelihood  و Bayesian ارزیابی گردید. حاصل این مطالعه، شناسایی 32 گونه از سیانوباکتری‌ها بود که در چهار راسته مختلف قرار ‌‌گرفت. راسته Oscillatoriales با سه تیره، هشت جنس و 13 گونه و راسته Chroococcales با دو تیره، سه جنس و چهار گونه به ترتیب از بیشترین و کمترین سطح تنوع برخوردار بودند. همچنین نتایج حاصل از این تحقیق، نشانگر کارآیی نسبی مارکر فیلوژنتیک (سکانس ژنی 16S rRNA) به کارگرفته شده و نیز کارایی مطالعات تاکسونومی عددی (آنالیز تجزیه خوشه‌ای) در جداسازی آرایه‌های مورد مطالعه بود. ارزیابی چگونگی پراکنش گونه‌های شناسایی شده در ایستگاه‌های مطالعاتی و ارایه شرح آرایه‌های جدید برای فلور جلبکی ایران از دیگر یافته‌های این مطالعه است. از میان آرایه‌های شناسایی شده، هفت گونه به اسامیO. ornata, Oscillatoria engelmanniana, Jaaginema pallidum, Pseudanabaena minima, Nostoc edaphicum, Gloeothece tepidariorum و Stenomitos frigidus برای نخستین بار از ایران گزارش می‌شوند.

Keywords [Persian]

  • آنالیز فیلوژنتیک
  • تنوع مورفولوژیکی
  • سیانوباکتری
  • فلزات سنگین
  • گونه جدید
  • 16S rRNA

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Rostaniha
Volume 18, Issue 2 - Serial Number 54
December 2017
Pages 107-121

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History

  • Receive Date: 08 April 2017
  • Revise Date: 10 March 2018
  • Accept Date: 19 June 2017

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