TY - JOUR ID - 101553 TI - Genetic diversity of Neotyphodium fungal endophytes in three Iranian grass species using AFLP molecular markers JO - Rostaniha JA - BOT.J.IRAN LA - en SN - 1608-4306 AU - KARIMI, S. AU - MIRLOHI, A.F. AU - SAYED TABATABAEI, B.E. AU - SHARIFNABI, B. AD - College of Agriculture, Isfahan University of Technology AD - College of Agriculture, Isfahan University of Technology E-mail: sharifna@cc.iut.ac.ir Y1 - 2009 PY - 2009 VL - 10 IS - 1 SP - 37 EP - 48 KW - Grass KW - Endophyte KW - Neotyphodium KW - Festuca KW - symbiosis KW - AFLP DO - N2 - Genetic diversity of fungal endophytes, Neotyphodium species, was studied in grasses Festuca arundinacea, F. pratensis and Lolium perenne using AFLP  markers. Fungi were isolated from the host leaf sheaths and Neotyphodium species were selected based on morphological characteristics. To confirm identity of selected fungi belonging to the genus Neotyphodium, polymerase chain reaction was performed using specific primers. AFLP with eight primer combinations was conducted to assess genetic variation for endophyte isolates. Cluster analysis based on AFLP data placed isolates into three separate groups according to their host species.  UR - https://rostaniha.areeo.ac.ir/article_101553.html L1 - https://rostaniha.areeo.ac.ir/article_101553_ae20b63b463746daec88f1ef6e35f71f.pdf ER -